Tested Applications
| Positive WB detected in | THP-1 cells, mouse heart tissue, mouse liver tissue, mouse lung tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human placenta tissue, mouse lung tissue, mouse small intestine tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20459-1-AP targets ZIP8 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14292 Product name: Recombinant human SLC39A8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 18-143 aa of BC012125 Sequence: LGGVAEGPGLAFSEDVLSVFGANLSLSAAQLQHLLEQMGAASRVGVPEPGQLHFNQCLTAEEIFSLHGFSNATQITSSKFSVICPAVLQQLNFHPCEDRPKHKTRPSHSEVWGYGFLSVTIINLAS Predict reactive species |
| Full Name | solute carrier family 39 (zinc transporter), member 8 |
| Calculated Molecular Weight | 460 aa, 50 kDa |
| Observed Molecular Weight | 42-46 kDa, 75-90 kDa,150 kDa |
| GenBank Accession Number | BC012125 |
| Gene Symbol | ZIP8 |
| Gene ID (NCBI) | 64116 |
| RRID | AB_10697830 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C0K1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC39A8, also known as ZIP8, belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that ZIP8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZIP8 antibody 20459-1-AP | Download protocol |
| IP protocol for ZIP8 antibody 20459-1-AP | Download protocol |
| WB protocol for ZIP8 antibody 20459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-ZIP8 antibody (Cat# 20459-1-AP) has 57 citations across journals including Cell, Nature Communications, JCI, Free Radical Biology and Medicine, Redox Biology, iScience, FASEB Journal, and Metallomics. Research fields span ferroptosis, metal ion homeostasis (zinc, manganese, iron, cadmium), inflammation, cardiovascular disease, neurodegeneration, reproductive toxicity, cancer, kidney/liver disease, pulmonary fibrosis, and bone tissue engineering.
| Species | Application | Title |
|---|---|---|
Nat Commun The manganese transporter SLC39A8 links alkaline ceramidase 1 to inflammatory bowel disease | ||
Redox Biol Identification of ZIP8-induced ferroptosis as a major type of cell death in monocytes under sepsis conditions | ||
Cardiovasc Diabetol Inhibition of Slc39a14/Slc39a8 reduce vascular calcification via alleviating iron overload induced ferroptosis in vascular smooth muscle cells | ||
J Clin Invest Hepatic metal ion transporter ZIP8 regulates manganese homeostasis and manganese-dependent enzyme activity.
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