Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20549-1-AP targets TOP2B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | topoisomerase (DNA) II beta 180kDa |
| Calculated Molecular Weight | 183 kDa |
| Observed Molecular Weight | 183 kDa |
| GenBank Accession Number | NM_001068 |
| Gene Symbol | TOP2B |
| Gene ID (NCBI) | 7155 |
| RRID | AB_10700004 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q02880 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TOP2B antibody 20549-1-AP | Download protocol |
| IHC protocol for TOP2B antibody 20549-1-AP | Download protocol |
| IP protocol for TOP2B antibody 20549-1-AP | Download protocol |
| WB protocol for TOP2B antibody 20549-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TOP2B Polyclonal antibody (Cat# 20549-1-AP) has 17 citations spanning journals including Science, Molecular Cell, Science Advances, Cell Death & Disease, Cell Reports, Journal of Biological Chemistry, RNA, Scientific Reports, Journal of Medicinal Chemistry, European Journal of Medicinal Chemistry, International Journal of Molecular Sciences, Toxicological Sciences, Naunyn-Schmiedeberg's Archives of Pharmacology, Cancer Treatment & Research Communications, and PLoS ONE. Research fields cover chromosome biology, oncology, DNA damage repair, drug development, ferroptosis, and transcription regulation.
| Species | Application | Title |
|---|---|---|
Science Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation. | ||
Mol Cell The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex | ||
Sci Adv XPF interacts with TOP2B for R-loop processing and DNA looping on actively transcribed genes | ||
Cell Death Dis MDM2 provides TOP2 poison resistance by promoting proteolysis of TOP2βcc in a p53-independent manner | ||
Cell Rep Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The reviewer used the antibody for Western Blot on HEK293 cells at a 1:1000 dilution and reported that it performed very well, clearly recognizing a band corresponding to TOP2B. Overall, the feedback indicates strong specificity and reliable performance in its tested application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Daniel (Verified Customer) (02-24-2025) | Antibody works really well and recognizes a band corresponding to TOP2B.
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