Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20595-1-AP targets POLR1A in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14543 Product name: Recombinant human RPA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 295-574 aa of BC017115 Sequence: DDEDMQEERNPHREGARKTQEQDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGGTGLFELKQPLR Predict reactive species |
| Full Name | polymerase (RNA) I polypeptide A, 194kDa |
| Calculated Molecular Weight | 1720 aa, 195 kDa |
| Observed Molecular Weight | 190-195 kDa |
| GenBank Accession Number | BC017115 |
| Gene Symbol | POLR1A |
| Gene ID (NCBI) | 25885 |
| RRID | AB_10949227 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95602 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for POLR1A antibody 20595-1-AP | Download protocol |
| WB protocol for POLR1A antibody 20595-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POLR1A Polyclonal antibody (Cat# 20595-1-AP) has 8 citations spanning journals including Cell, Redox Biology, Nucleic Acids Research, EMBO Reports, Cancer Science, Journal of Neuroscience, Clinical and Translational Medicine, and Research. Research fields covered include DNA double-strand break repair, ferroptosis and mitophagy, rRNA processing, ribosome biogenesis, rDNA transcription, proteogenomic cancer subtyping, and activity-dependent rRNA biogenesis. Applications cited include WB, IP, and ChIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell RNA polymerase III is required for the repair of DNA double-strand breaks by homologous recombination. | ||
Redox Biol POLR1A inhibits ferroptosis by regulating TFAM-mediated mitophagy and iron homeostasis.
| ||
Research (Wash D C) Proteogenomic Analysis Identifies Clinically Relevant Subgroups of Collecting Duct Carcinoma | ||
Clin Transl Med PCDHGB7 inhibits the progression of triple-negative breast cancer by suppressing XRCC5/MYC-mediated ribosome biogenesis. | ||
EMBO Rep Kin17 promotes rDNA transcription, ribosomal biogenesis, and cortical lamination. |
Reviews
What customers say
The single review for this product is a 5-star rating for Western Blot. The reviewer used a 1:1000 dilution on HeLa cell protein extract (5 micrograms) and included an image of the resulting blot. Overall, the feedback is positive, indicating the antibody performed well in WB under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anita (Verified Customer) (12-22-2020) | HeLa cell protein extract (5micrograms)
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