Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20702-1-AP targets IRS2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | IRS2 |
| Calculated Molecular Weight | 137 kDa |
| Observed Molecular Weight | 150-185 kDa |
| GenBank Accession Number | NM_003749 |
| Gene Symbol | IRS2 |
| Gene ID (NCBI) | 8660 |
| RRID | AB_2918067 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4H2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The family of INS receptor substrates (IRS) consists of four proteins (IRS-1-IRS-4), which were initially characterized as typical cytosolic adaptor proteins involved in INS receptor (IR) and INS-like growth factor I receptor (IGF-IR) signaling. Impaired glucose tolerance and INS resistance, and thus type 2 diabetes were demonstrated in Irs2 knockout mice. In vivo data also indicated that he regulation of beta-cell mass involves IRS2, while IRS1 is only required for proper INS production in beta-cells. IRS2, like IRS1, has a predicted molecular weight of 137 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS-PAGE as a band of approximately 150-185 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IRS2 antibody 20702-1-AP | Download protocol |
| WB protocol for IRS2 antibody 20702-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-IRS2 antibody (Cat# 20702-1-AP) has 15 total citations published in journals including Int J Biol Sci, Biochem Pharmacol, Sci Rep, Diabetol Metab Syndr, Heliyon, Obesity, Pediatr Res, Brain Sci, Food Funct, and Am J Transl Res. Associated research fields span type 2 diabetes, gestational diabetes, insulin resistance, glucose homeostasis, PI3K/AKT signaling, cancer progression, neuroprotection, and mitochondrial metabolism.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci CircFAT1 Promotes Lung Adenocarcinoma Progression by Sequestering miR-7 from Repressing IRS2-ERK-mediated CCND1 Expression. | ||
Oxid Med Cell Longev Microbiota from Exercise Mice Counteracts High-Fat High-Cholesterol Diet-Induced Cognitive Impairment in C57BL/6 Mice | ||
Biochem Pharmacol A novel covalent inhibitor alleviates type 2 diabetes mellitus by restraining GSK-3β | ||
Food Funct Elucidation of the interaction effect between dietary fiber and bound polyphenol components on the anti-hyperglycemic activity of tea residue dietary fiber. | ||
Diabetol Metab Syndr IL-37 inhibited inflammation to improve gestational diabetes mellitus through the GSK3/NF-κB pathway. | ||
Sci Rep TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis. |
