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What's the best dilution for PRKD1 in ?
Tested Applications
| Positive WB detected in | LNCaP cells, DU 145 cells, HEK-293 cells |
| Positive IHC detected in | rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20714-1-AP targets PRKD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | protein kinase D1 |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | NM_002742 |
| Gene Symbol | PRKD1 |
| Gene ID (NCBI) | 5587 |
| RRID | AB_3669341 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15139 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PRKD1 antibody 20714-1-AP | Download protocol |
| WB protocol for PRKD1 antibody 20714-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PRKD1 Polyclonal antibody (Cat# 20714-1-AP) has 3 citations. These appear in Advanced Science, European Journal of Pharmacology, and Applied Biochemistry and Biotechnology. The research fields span tenosynovial giant cell tumor pathology and biomarker identification, neuroprotection in acute spinal cord injury via oxidative stress modulation, and chemoprevention of mammary cancer through PI3K/AKT/mTOR pathway analysis. Applications include IHC, WB, and IF in human and rat samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Single-Cell Analysis Clarifies Pathological Heterogeneity in Tenosynovial Giant Cell Tumor and Identifies Biomarkers for Predicting Disease Recurrence | ||
Eur J Pharmacol Tetramethylpyrazine exerts a neuroprotective effect in acute spinal cord injury by mitigating oxidative stress through PKD1: multi-omics analysis and experimental validation | ||
Appl Biochem Biotechnol Chemopreventive Potential of Brucine-Gold Nanoparticles (BRU-AuNPs) in DMBA-induced Mammary Cancer via Modulation of PI3K/AKT/mTOR Pathway. |
