Tested Applications
| Positive WB detected in | COLO 320 cells, human skeletal muscle tissue, mouse colon tissue, HEK-293 cells, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20716-1-AP targets MYH14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, frog |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 14 |
| Calculated Molecular Weight | 228 kDa |
| Observed Molecular Weight | 228 kDa |
| GenBank Accession Number | NM_024729 |
| Gene Symbol | MYH14 |
| Gene ID (NCBI) | 79784 |
| RRID | AB_10859247 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z406 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH14 antibody 20716-1-AP | Download protocol |
| IHC protocol for MYH14 antibody 20716-1-AP | Download protocol |
| IP protocol for MYH14 antibody 20716-1-AP | Download protocol |
| WB protocol for MYH14 antibody 20716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYH14 Polyclonal antibody (Cat# 20716-1-AP) has 21 citations published in journals including Advanced Science, Science Advances, Current Biology, Journal of Cell Biology, Molecular Biology of the Cell, Science Signaling, Communications Biology, and others. Research fields span non-muscle myosin biology, cytokinesis and cell shape regulation, cochlear hair cell function and deafness, intestinal microvilli and barrier integrity, stem cell differentiation, muscular dystrophy, podocyte injury, and connective tissue disease.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Microfilament-Myosin II Regulates the Differentiation of Multinucleated Cysts into Oocytes and Influences Oocyte Developmental Potential in Mice. | ||
Sci Adv Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions. | ||
Int J Nanomedicine Combining Porous Se@SiO2 Nanocomposites and dECM Enhances the Myogenic Differentiation of Adipose-Derived Stem Cells | ||
Curr Biol Spef1 is a microvillar component that limits apical actomyosin contractility and preserves intestinal barrier function. | ||
