Tested Applications
| Positive WB detected in | HT-29 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20727-1-AP targets PROM2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | prominin 2 |
| Calculated Molecular Weight | 92 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | NM_144707 |
| Gene Symbol | PROM2 |
| Gene ID (NCBI) | 150696 |
| RRID | AB_3085619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N271 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PROM2, also named as prominin 2, encodes a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants. PROM2 has 2 isoforms with the MW of 86 kDa and 41 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PROM2 antibody 20727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PROM2 Polyclonal antibody (Cat# 20727-1-AP) has 1 total citation. It appears in Phytotherapy Research (IF 8.1), published in 2026. The cited study investigated Z-guggulsterone-induced ferroptosis in chemoresistant breast cancer via CDKN2B-mediated suppression of GTP cyclohydrolase 1, using this antibody for Western blot in human samples. The associated research fields include ferroptosis and breast cancer.
| Species | Application | Title |
|---|---|---|
Phytother Res Z-Guggulsterone Induces Ferroptosis in Chemoresistant Breast Cancer via a CDKN2B-Mediated Suppression of GTP Cyclohydrolase 1. |
