Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, Jurkat cells, rat brain tissue |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21026-1-AP targets TRIM46 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15290 Product name: Recombinant human TRIM46 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC069416 Sequence: MAEGEDMQTFTSIMDALVRISTSMKNMEKELLCPVCQEMYKQPLVLPCTHNVCQACAREVLGQQGYIGHGGDPSSEPTSPASTPSTRSPRLSRRTLPKPDRLDRLLKSGFGTYPGRKRGALHPQVIMFPCPACQGDVELGERGLAGLFRNLTLERVVERYRQSVSVGGAILCQLCKPPPLEATKGCTECRATFCNECFKLFHPWGTQKAQHEPTLPTLSFRPKGLMCPDHKEEVTHYCKTCQRLVCQLCRVRRTHSGHKITPVLSAYQALKDKLTKSLTYILGNQDTVQTQICELEEAVRHTEVSGQQAKEEVSQLVRGLGAVLEEKRASLLQAIEECQQERLARLSAQI Predict reactive species |
| Full Name | tripartite motif-containing 46 |
| Calculated Molecular Weight | 759 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC069416 |
| Gene Symbol | TRIM46 |
| Gene ID (NCBI) | 80128 |
| RRID | AB_10732843 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z4K8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM46 antibody 21026-1-AP | Download protocol |
| WB protocol for TRIM46 antibody 21026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-TRIM46 (CASM) antibody (Cat# 21026-1-AP) has 13 citations published in journals including Nature Communications, Autophagy, Cell Reports, Journal of Cell Biology, FASEB Journal, International Journal of Molecular Sciences, Oncology Research, Experimental Cell Research, American Journal of Cancer Research, Eye Vision, Journal of Pharmacological Sciences, Developmental Dynamics, and bioRxiv. Research fields span axon initial segment formation, Golgi repair, ferroptosis, breast/ovarian/gastric cancer, osteosarcoma, retinal inflammation, and neuronal architecture.
| Species | Application | Title |
|---|---|---|
Nat Commun Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation. | ||
Cell Rep The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling. | ||
J Cell Biol The KIF3B/B/KAP3 tail domain specifically facilitates TRIM46 transport to the axon initial segment. | ||
Int J Mol Sci Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46. | ||
Eye Vis (Lond) TRIM46 aggravated high glucose-induced hyper permeability and inflammatory response in human retinal capillary endothelial cells by promoting IκBα ubiquitination
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