Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21088-1-AP targets GCM2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15334 Product name: Recombinant human GCM2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 157-506 aa of BC069603 Sequence: HDHPRPESKSETEARRSAIKRQMASFYQPQKKRIRESEAEENQDSSGHFSNIPPLENPEDFDIVTETSFPIPGQPCPSFPKSDVYKATCDLATFQGDKMPPFQKYSSPRIYLPRPPCSYELANPGYTNSSPYPTLYKDSTSIPNDTDWVHLNTLQCNVNSYSSYERSFDFTNKQHGWKPALGKPSLVERTNHGQFQAMATRPYYNPELPCRYLTTPPPGAPALQTVITTTTKVSYQAYQPPAMKYSDSVREVKSLSSCNYAPEDTGMSVYPEPWGPPVTVTRAASPSGPPPMKIAGDCRAIRPTVAIPHEPVSSRTDEAETWDVCLSGLGSAVSYSDRVGPFFTYNNEDF Predict reactive species |
| Full Name | glial cells missing homolog 2 (Drosophila) |
| Calculated Molecular Weight | 506 aa, 57 kDa |
| Observed Molecular Weight | 50-56 kDa |
| GenBank Accession Number | BC069603 |
| Gene Symbol | GCM2 |
| Gene ID (NCBI) | 9247 |
| RRID | AB_10733096 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75603 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GCM2 antibody 21088-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GCM2 polyclonal antibody (Cat# 21088-1-AP) has 2 total citations. These appear in Artificial Organs and Biosciences Reports. Both studies focus on parathyroid biology, specifically parathyroid organoid construction methods and serum-free culture conditions for maintaining primary parathyroid cells in vitro.
| Species | Application | Title |
|---|---|---|
Artif Organs Comparison of the Construction Methods of Parathyroid Organoids and Functional Influence of Calcium on Organoids | ||
Biosci Rep Serum-free culture with PVP enables maintenance of primary culture of parathyroid cells in vitro. |
