KIAA0528 Polyclonal antibody

KIAA0528 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21138-1-AP

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C2 domain-containing phosphoprotein of 138 kDa, C2 domain-containing protein 5, C2CD5, CDP138

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 21138-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 21138-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 21138-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 21138-1-AP
IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse small intestine using 21138-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse small intestine using 21138-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse lung using 21138-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 21138-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 21138-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 21138-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 21138-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 21138-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 21138-1-AP
Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for KIAA0528 in ?

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse small intestine tissue, mouse lung tissue, human ovary cancer tissue, mouse ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21138-1-AP targets KIAA0528 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15590 Product name: Recombinant human KIAA0528 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 651-1000 aa of BC042498 Sequence: RSRLLRSQSESSDEVTELDLSHGKKDAFVLEIDDTDAMEDVHSLLTDVPPPSGFYSCNTEIMPGINNWTSEIQMFTSVRVIRLSSLNLTNQALNKNFNDLCENLLKSLYFKLRSMIPCCLCHVNFTVSLPEDELIQVTVTAVAITFDKNQALQTTKTPVEKSLQRASTDNEELLQFPLELCSDSLPSHPFPPAKAMTVEKASPVGDGNFRNRSAPPCANSTVGVVKMTPLSFIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIAEVFAMVRAHVAALGGNAVVSYIMKQCVFMENPNKNQAQCLINVSGDAVVFVRESDLEVVSSQQPTTNCQSSCTEGEVTT Predict reactive species
Full Name	KIAA0528
Calculated Molecular Weight	1000 aa, 110 kDa
Observed Molecular Weight	110-125 kDa
GenBank Accession Number	BC042498
Gene Symbol	KIAA0528
Gene ID (NCBI)	9847
RRID	AB_10858480
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86YS7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for KIAA0528 antibody 21138-1-AP	Download protocol
IHC protocol for KIAA0528 antibody 21138-1-AP	Download protocol
IP protocol for KIAA0528 antibody 21138-1-AP	Download protocol
WB protocol for KIAA0528 antibody 21138-1-AP	Download protocol