Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, mouse heart, rat heart |
| Positive IP detected in | mouse liver tissue |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21147-1-AP targets TACO1/CCDC44 in WB, IHC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15688 Product name: Recombinant human CCDC44 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC007744 Sequence: MSAWAAASLSRAAARCLLARGPGVRAAPPRDPRPSHPEPRGCGAAPGRTLHFTAAVPAGHNKWSKVRHIKGPKDVERSRIFSKLCLNIRLAVKEGGPNPEHNSNLANILEVCRSKHMPKSTIETALKMEKSKDTYLLYEGRGPGGSSLLIEALSNSSHKCQADIRHILNKNGGVMAVGARHSFDKKGVIVVEVEDREKKAVNLERALEMAIEAGAEDVKETEDEEERNVFKFICDASSLHQVRKKLDSLGLCSVSCALEFIPNSKVQLAEPDLEQAAHLIQALSNHEDVIHVYDNIE Predict reactive species |
| Full Name | coiled-coil domain containing 44 |
| Calculated Molecular Weight | 297 aa, 32 kDa |
| Observed Molecular Weight | 30-32 kDa |
| GenBank Accession Number | BC007744 |
| Gene Symbol | TACO1 |
| Gene ID (NCBI) | 51204 |
| RRID | AB_10732604 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BSH4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TACO1 (translational activator of cytochrome oxidase subunit I) is required for COXI translation. Mutations in TACO1 cause Leigh syndrome (LS) and cytochrome c oxidase deficiency. (27319982)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TACO1/CCDC44 antibody 21147-1-AP | Download protocol |
| IHC protocol for TACO1/CCDC44 antibody 21147-1-AP | Download protocol |
| IP protocol for TACO1/CCDC44 antibody 21147-1-AP | Download protocol |
| WB protocol for TACO1/CCDC44 antibody 21147-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TACO1/CCDC44 Polyclonal antibody (catalog number 21147-1-AP) has 12 citations. These appear in journals including Cell, Nature Cell Biology, Nature Communications, Advanced Science, Science Advances, EMBO Journal, Free Radical Biology and Medicine, Cell Reports, Experimental Cell Research, Biochemical and Biophysical Research Communications, Physiological Reports, and bioRxiv. The associated research fields span mitochondrial translation, oxidative phosphorylation, muscle differentiation, mitochondrial biogenesis, cancer biology (bladder and gastric cancer), lipid metabolism, and stemness.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression | ||
Nat Commun Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.
| ||
Adv Sci (Weinh) Enhanced Oxidative Phosphorylation Driven by TACO1 Mitochondrial Translocation Promotes Stemness and Cisplatin Resistance in Bladder Cancer | ||
Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. | ||
EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. |
