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TMEM117 Polyclonal antibody

TMEM117 Polyclonal Antibody for WB, ELISA

Cat No. 21314-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

TMEM117, transmembrane protein 117

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of HepG2 using 21314-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21314-1-AP (TMEM117 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MDA-MB-453s using 21314-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 21314-1-AP (TMEM117 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HepG2 cells, MDA-MB-453s cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21314-1-AP targets TMEM117 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15966 Product name: Recombinant human TMEM117 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-410 aa of BC030284 Sequence: GIIFLVLILDLNMWKNQIFYKPHEYGQYIGPGQKIYTVKDSESLKDLNRTKLSWEWRSNHTNPRTNKTYVEGDMFLHSRFIGASLDVKCLAFVPSLIAFVWFGFFIWFFGRFLKNEPRMENQDKTYTRMKRKSPSEHSKDMGITRENTQASVEDPLNDPSLVCIRSDFNEIVYKSSHLTSENLSSQLNESTSATEADQDPTTSKSTPTN Predict reactive species
Full Name	transmembrane protein 117
Calculated Molecular Weight	514 aa, 60 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC030284
Gene Symbol	TMEM117
Gene ID (NCBI)	84216
RRID	AB_2878838
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H0C3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TMEM117 antibody 21314-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The TMEM117 Polyclonal antibody (Cat# 21314-1-AP) has 1 citation. It was published in Frontiers in Oncology (IF 3.4, 2021), where it was used for WB and IF in human samples. The study investigated how a class of protein-coding RNAs binds to Polycomb Repressive Complex 2 and alters histone methylation, placing this citation in the fields of oncology, epigenetics, and non-coding/protein-coding RNA biology.

Species	Application	Title
human	WB,IF	Front Oncol A Class of Protein-Coding RNAs Binds to Polycomb Repressive Complex 2 and Alters Histone Methylation. Authors - Meijian Liao KD Validated View Article