Tested Applications
| Positive WB detected in | SW 1990 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21336-1-AP targets MTMR3 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15514 Product name: Recombinant human MTMR3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 682-1031 aa of BC151217 Sequence: AGVAEGQMENILQEATKEESGVEEPAHRAGIEIQEGKEDPLLEKESRRKTPEASAIGLHQDPELGDAALRSHLDMSWPLFSQGISEQQSGLSVLLSSLQVPPRGEDSLEVPVEQFRIEEIAEGREEAVLPIPVDAKVGYGTSQSCSLLPSQVPFETRGPNVDSSTDMLVEDKVKSVSGPQGHHRSCLVNSGKDRLPQTMEPSPSETSLVERPQVGSVVHRTSLGSTLSLTRSPCALPLAECKEGLVCNGAPETENRASEQPPGLSTLQMYPTPNGHCANGEAGRSKDSLSRQLSAMSCSSAHLHSRNLHHKWLHSHSGRPSATSSPDQPSRSHLDDDGMSVYTDTIQQRL Predict reactive species |
| Full Name | myotubularin related protein 3 |
| Calculated Molecular Weight | 1198 aa, 134 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC151217 |
| Gene Symbol | MTMR3 |
| Gene ID (NCBI) | 8897 |
| RRID | AB_2878842 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13615 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MTMR3 antibody 21336-1-AP | Download protocol |
| WB protocol for MTMR3 antibody 21336-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTMR3 Polyclonal antibody (Cat# 21336-1-AP) has 2 total citations, appearing in Molecular Therapy Nucleic Acids and Journal of Cell Biology. The cited studies cover research in glioblastoma progression involving m6A-dependent miRNA processing and KRAS signaling regulated by plasma membrane phospholipid control, respectively. Both citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Mol Ther Nucleic Acids SPI1-induced downregulation of FTO promotes GBM progression by regulating pri-miR-10a processing in an m6A-dependent manner. | ||
J Cell Biol MTMR regulates KRAS function by controlling plasma membrane levels of phospholipids.
|
