Tested Applications
| Positive WB detected in | HL-60 cells, Jurkat cells; K-562 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21354-1-AP targets WDR82 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15762 Product name: Recombinant human WDR82 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 175-313 aa of BC018941 Sequence: DLRSFDKGPFATFKMQYDRTCEWTGLKFSNDGKLILISTNGSFIRLIDAFKGVVMHTFGGYANSKAVTLEASFTPDSQFIMIGSEDGKIHVWNGESGIKVAVLDGKHTGPITCLQFNPKFMTFASACSNMAFWLPTIDD Predict reactive species |
| Full Name | WD repeat domain 82 |
| Calculated Molecular Weight | 313 aa, 35 kDa |
| Observed Molecular Weight | 33-35 kDa |
| GenBank Accession Number | BC018941 |
| Gene Symbol | WDR82 |
| Gene ID (NCBI) | 80335 |
| RRID | AB_3085654 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UXN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for WDR82 antibody 21354-1-AP | Download protocol |
| WB protocol for WDR82 antibody 21354-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The WDR82 Polyclonal antibody (catalog number 21354-1-AP) has 2 total citations. These appear in the Journal of Experimental & Clinical Cancer Research and Science Advances. The cited research spans oncology and stem cell biology: one study examined radiotherapy resistance in breast cancer driven by asparagine endopeptidase through ATR pathway modulation, while the other investigated EIF3D's role in maintaining signaling pathway homeostasis during human primed pluripotency. Both applications used Western blotting in human samples.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Radiotherapy resistance driven by Asparagine endopeptidase through ATR pathway modulation in breast cancer | ||
Sci Adv EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency |
Reviews
What customers say
The single review rates the antibody 5 stars. It was used for immunohistochemistry and immunofluorescence on mouse and human prostate tissue and BHPrS1 cells. At a 1:100 dilution for staining BHPrS1 cells, the reviewer plans to reduce it to 1:500. For IHC of mouse and human prostate tissue at 1:200, it performed well. Overall, the antibody was described as highly effective and reliable for the intended applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mia (Verified Customer) (07-01-2025) | This antibody was incredibly helpful for my summer project while in medical school. It was used at a 1:100 dilution for staining BHPrS1 cells, and we are planning to drop the dilution to 1:500. It was also used at a 1:200 dilution for IHC of mouse and human prostate tissue, and worked wonderfully.
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