Tested Applications
| Positive IP detected in | MCF-7 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21375-1-AP targets ERMAP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15899 Product name: Recombinant human ERMAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 176-475 aa of BC099703 Sequence: IWKQRRAKEKLLYEHVTEVDNLLSDHAKEKGKLHKAVKKLRSELKLKRAAANSGWRRARLHFVAVTLDPDTAHPKLILSEDQRCVRLGDRRQPVPDNPQRFDFVVSILGSEYFTTGCHYWEVYVGDKTKWILGVCSESVSRKGKVTASPANGHWLLRQSRGNEYEALTSPQTSFRLKEPPRCVGIFLDYEAGVISFYNVTNKSHIFTFTHNFSGPLRPFFEPCLHDGGKNTAPLVICSELHKSEESIVPRPEGKGHANGDVSLKVNSSLLPPKAPELKDIILSLPPDLGPALQELKAPSF Predict reactive species |
| Full Name | erythroblast membrane-associated protein (Scianna blood group) |
| Calculated Molecular Weight | 475 aa, 53 kDa |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC099703 |
| Gene Symbol | ERMAP |
| Gene ID (NCBI) | 114625 |
| RRID | AB_2878849 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PL5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ERMAP antibody 21375-1-AP | Download protocol |
| IP protocol for ERMAP antibody 21375-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ERMAP polyclonal antibody (Cat# 21375-1-AP) has 1 citation to date. It was cited in Nature Immunology (IF 26.5) in a 2023 study by Jie Li et al., which investigated how the interaction between ERMAP, galectin-9, and dectin-2 promotes Kupffer cell phagocytosis and antitumor immunity in mice. The antibody was used for Western blotting in this research, placing it within the fields of innate immunity, macrophage biology, and tumor immunology.
| Species | Application | Title |
|---|---|---|
Nat Immunol The ligation between ERMAP, galectin-9 and dectin-2 promotes Kupffer cell phagocytosis and antitumor immunity |
