Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21557-1-AP targets LAX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15599 Product name: Recombinant human LAX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-322 aa of BC069650 Sequence: MPLLTLPQTRQRAKNIYDILPWRQEDLGRHESRSMRIFSTESLLSRNSESPEHVPSQAGNAFQEHTAHIHATEYAVGIYDNAMVPQMCGNLTPSAHCINVRASRDCASISSEDSHDYVNVPTAEEIAETLASTKSPSRNLFVLPSTQKLEFTEERDEGCGDAGDCTSLYSPGAEDSDSLSNGEGSSQISNDYVNMTGLDLSAIQERQLWVAFQCCRDYENVPAADPSGSQQQAEKDVPSSNIGHVEDKTDDPGTHVQCVKRTFLASGDYADFQPFTQSEDSQMKHREEMSNEDSSDYENVLTAKLGGRDSEQGPGTQLLPDE Predict reactive species |
| Full Name | lymphocyte transmembrane adaptor 1 |
| Calculated Molecular Weight | 398 aa, 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC069650 |
| Gene Symbol | LAX1 |
| Gene ID (NCBI) | 54900 |
| RRID | AB_10732821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWV1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LAX1 antibody 21557-1-AP | Download protocol |
| IHC protocol for LAX1 antibody 21557-1-AP | Download protocol |
| WB protocol for LAX1 antibody 21557-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LAX1 Polyclonal antibody (Cat# 21557-1-AP) has 3 total citations. These appear in Cell Reports in Medicine, BMC Geriatrics, and Journal of Immunology. The associated research fields include immunotherapy for multiple myeloma, Alzheimer's disease and periodontitis biomarker research, and m6A RNA modification in germinal center B cell responses. All three citations used the antibody for Western blot (WB) applications in human or mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Med A target discovery pipeline identified ILT3 as a target for immunotherapy of multiple myeloma | ||
BMC Geriatr LAX1 as a core biomarker in Alzheimer's disease and periodontitis via the STAT signaling pathway.
| ||
J Immunol Mettl14-Mediated m6A Modification Is Essential for Germinal Center B Cell Response. |
