Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat small intestine tissue, mouse brain tissue, mouse intestine Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21649-1-AP targets SLITRK3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16352 Product name: Recombinant human SLITRK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 745-964 aa of BC114621 Sequence: HPVTQMCNNPIYKPREEEEVAVSSAQEAGSAERGGPGTQPPGMGEALLGSEQFAETPKENHSNYRTLLEKEKEWALAVSSSQLNTIVTVNHHHPHHPAVGGVSGVVGGTGGDLAGFRHHEKNGGVVLFPPGGGCGSGSMLLDRERPQPAPCTVGFVDCLYGTVPKLKELHVHPPGMQYPDLQQDARLKETLLFSAGKGFTDHQTQKSDYLELRAKLQTKP Predict reactive species |
| Full Name | SLIT and NTRK-like family, member 3 |
| Calculated Molecular Weight | 977 aa, 109 kDa |
| Observed Molecular Weight | 120-125 kDa |
| GenBank Accession Number | BC114621 |
| Gene Symbol | SLITRK3 |
| Gene ID (NCBI) | 22865 |
| RRID | AB_10859788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94933 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SLITRK3 antibody 21649-1-AP | Download protocol |
| IP protocol for SLITRK3 antibody 21649-1-AP | Download protocol |
| WB protocol for SLITRK3 antibody 21649-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SLITRK3 polyclonal antibody (Cat# 21649-1-AP) has 5 total citations published in Neuron, Molecular Biomedicine, PNAS, Frontiers in Molecular Neuroscience, and The Journal of Neuroscience. Research fields include GABAergic synapse development, squamous cell lung cancer, excitatory and inhibitory synapse formation with LAR receptor tyrosine phosphatases, and the role of Neuroligin 2 in OCD and social behavior. Applications cited include WB, IHC, and IF across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Neuron Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development. | ||
Mol Biomed Role of novel cancer gene SLITRK3 to activate NTRK3 in squamous cell lung cancer.
| ||
Proc Natl Acad Sci U S A Slitrks control excitatory and inhibitory synapse formation with LAR receptor protein tyrosine phosphatases.
| ||
Front Mol Neurosci Human mutations in SLITRK3 implicated in GABAergic synapse development in mice | ||
