Tested Applications
| Positive WB detected in | BxPC-3 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse colon tissue, human placenta tissue, human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21738-1-AP targets Integrin beta 4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16450 Product name: Recombinant human Integrin beta-4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-354 aa of BC126411 Sequence: ANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSN Predict reactive species |
| Full Name | integrin, beta 4 |
| Calculated Molecular Weight | 1752 aa, 195 kDa |
| Observed Molecular Weight | 200-240 kDa |
| GenBank Accession Number | BC126411 |
| Gene Symbol | Integrin beta 4 |
| Gene ID (NCBI) | 3691 |
| RRID | AB_10733888 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16144 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Integrin beta 4 antibody 21738-1-AP | Download protocol |
| IHC protocol for Integrin beta 4 antibody 21738-1-AP | Download protocol |
| IP protocol for Integrin beta 4 antibody 21738-1-AP | Download protocol |
| WB protocol for Integrin beta 4 antibody 21738-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ITGB4 (Integrin Beta 4) antibody (Cat# 21738-1-AP) has 39 citations across journals including Cell Host & Microbe, Nature Communications, Oncogene, Cell Death & Disease, FASEB Journal, Journal of Biological Chemistry, and Phytomedicine. Research fields span oncology (lung, bladder, gastric, pancreatic, breast, colorectal, and cervical cancers), EMT, metastasis, immune evasion, liver fibrosis, cell adhesion, and integrin/FAK/MAPK/TGF-β signaling pathways.
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Peptostreptococcus stomatis promotes colonic tumorigenesis and receptor tyrosine kinase inhibitor resistance by activating ERBB2-MAPK
| ||
Nat Commun Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis. | ||
J Exp Clin Cancer Res Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.
| ||
MedComm (2020) Nicotinamide N-Methyltransferase Epigenetically Activates Fibronectin 1 Through H3K9me3 Remodeling in Clear Cell Renal Cell Carcinoma. | ||
Cell Death Dis SOAT1 regulates cholesterol metabolism to induce EMT in hepatocellular carcinoma | ||
Cell Commun Signal METTL14-mediated N6-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma.
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Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The user reported that the antibody recognized a protein at the expected size, indicating reliable specificity and performance for WB use. Overall, the feedback is positive, confirming the reagent works as intended for detecting the target at the correct molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (11-25-2024) | recognized a protein at the expected size
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