Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A2780 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21749-1-AP targets HIGD1A in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14027 Product name: Recombinant human HIGD1A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-93 aa of BC000601 Sequence: MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP Predict reactive species |
| Full Name | HIG1 hypoxia inducible domain family, member 1A |
| Calculated Molecular Weight | 93 aa, 10 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | BC000601 |
| Gene Symbol | HIGD1A |
| Gene ID (NCBI) | 25994 |
| RRID | AB_10858789 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y241 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for HIGD1A antibody 21749-1-AP | Download protocol |
| WB protocol for HIGD1A antibody 21749-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-HIGD1A antibody (Cat# 21749-1-AP) has 10 total citations published in journals including Advanced Science, PNAS, Oncogene, Cell Reports, Molecular & Cellular Proteomics, Cancer Metabolism, FASEB Journal, PLoS ONE, and Bioengineered. Research fields span mitochondrial biology, DNA damage response, cancer progression (hepatocellular and colorectal carcinoma), metabolic regulation, respiratory function, and protein profiling.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Affinity-Based Protein Profiling Revealed that HIGD1A is a Direct Target Protein of Aristolochic Acids.
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Proc Natl Acad Sci U S A Higd1a is a positive regulator of cytochrome c oxidase.
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Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.
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Cell Rep HIGD1A links SIRT1 activity to adipose browning by inhibiting the ROS/DNA damage pathway | ||
Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.
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Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV
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