ARHGAP27 Polyclonal antibody

ARHGAP27 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 22077-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SH3 domain-containing protein 20, Rho-type GTPase-activating protein 27, Rho GTPase-activating protein 27, PP905, CIN85-associated multi-domain-containing Rho GTPase-activating protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHCIF/ICC

WB analysis of A549 using 22077-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22077-1-AP (ARHGAP27 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 22077-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 22077-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas using 22077-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas using 22077-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 22077-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22077-1-AP (ARHGAP27 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for ARHGAP27 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	A549 cells
Positive IHC detected in	human breast cancer tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22077-1-AP targets ARHGAP27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17225 Product name: Recombinant human ARHGAP27 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-300 aa of BC101388 Sequence: MQPGLSPGSPGDPRPPTPETDYPESLTSYPEEDYSPVGSFGEPGPTSPLTTPPGWSCHVSQDKQMLYTNHFTQEQWVRLEDPHGKPYFYNPEDSSVRWELPQVPVPAPRSIHKSSQDGDTPAQASPPEEKVPAELDEVGSWEEVSPATAAVRTKTLDKAGVLHRTKTADKGKRLRKKHWSASWTVLEGGVLTFFKDSKTSAAGGLRQPSKFSTPEYTVELRGATLSWAPKDKSSRKNVLELRSRDGSEYLIQHDSEAIISTWHKAIAQGIQELSAELPPEESESSRVDFGSSERLGSWQE Predict reactive species
Full Name	Rho GTPase activating protein 27
Calculated Molecular Weight	548 aa, 62 kDa
Observed Molecular Weight	98 kDa
GenBank Accession Number	BC101388
Gene Symbol	ARHGAP27
Gene ID (NCBI)	201176
RRID	AB_2878985
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6ZUM4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ARHGAP27 antibody 22077-1-AP	Download protocol
IHC protocol for ARHGAP27 antibody 22077-1-AP	Download protocol
WB protocol for ARHGAP27 antibody 22077-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ARHGAP27 Polyclonal antibody (Cat# 22077-1-AP) has 1 total citation. It appears in iScience (Impact Factor 4.5), published in 2024. The cited study investigated targeting ALDOA to modulate tumorigenesis and energy metabolism in retinoblastoma, placing this citation in the fields of cancer biology and metabolic regulation. The antibody was used for Western blot (WB) in human samples.