SLAIN1 Polyclonal antibody

SLAIN1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 22123-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

C13orf32, SLAIN motif family, member 1, SLAIN motif-containing protein 1

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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Filter: AllWBIF/ICC

WB analysis of HeLa using 22123-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22123-1-AP (SLAIN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 22123-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22123-1-AP (SLAIN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 22123-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 22123-1-AP (SLAIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 22123-1-AP
Immunofluorescent analysis of HeLa cells using 22123-1-AP (SLAIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 22123-1-AP
Immunofluorescent analysis of HEK-293 cells using 22123-1-AP (SLAIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, mouse brain tissue
Positive IF/ICC detected in	HeLa cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22123-1-AP targets SLAIN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4973 Product name: Recombinant human SLAIN1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 60-364 aa of BC045177 Sequence: MGYKLQDLTDVQIMARLQEESLRQDYASTSASVSRHSSSVSLSSGKKGTCSDQEYDQYSLEDEEEFDHLPPPQPRLPRCSPFQRGIPHSQTFSSIRECRRSPSSQYFPSNNYQQQQYYSPQAQTPDQQPNRTNGDKLRRSMPNLARMPSTTAISSNISSPVTVRNSQSFDSSLHGAGNGISRIQSCIPSPGQLQHRVHSVGHFPVSIRQPLKATAYVSPTVQGSSNMPLSNGLQLYSNTGIPTPNKAAASGIMGRSALPRPSLAINGSNLPRSKIAQPVRSFLQPPKPLSSLSTLRDGNWRDGCY Predict reactive species
Full Name	SLAIN motif family, member 1
Calculated Molecular Weight	61 kDa
Observed Molecular Weight	65-70 kDa, 34-40 kDa
GenBank Accession Number	BC045177
Gene Symbol	SLAIN1
Gene ID (NCBI)	122060
RRID	AB_2878999
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8ND83
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The SLAIN1 Polyclonal antibody (Cat# 22123-1-AP) has 1 citation. It was used in a study published in Scientific Reports (2023) for Western blot analysis in human and mouse samples. The research focused on identifying potential biomarkers of idiopathic pulmonary fibrosis through machine learning analysis, placing it in the fields of pulmonary disease and bioinformatics.

Species	Application	Title
mouse,human	WB	Sci Rep Identifying potential biomarkers of idiopathic pulmonary fibrosis through machine learning analysis Authors - Zenan Wu View Article