Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, rat testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22140-1-AP targets RASAL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17555 Product name: Recombinant human RASAL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 609-958 aa of BC110611 Sequence: LRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDERESSLPNGRSVSLMDLQDTHAAQVEHASVMLDVPIRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGGLQPLSFQNPVYHLNNPIPAMPKASIDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFT Predict reactive species |
| Full Name | RAS protein activator like 2 |
| Calculated Molecular Weight | 1262 aa, 142 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC110611 |
| Gene Symbol | RASAL2 |
| Gene ID (NCBI) | 9462 |
| RRID | AB_2879005 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJF2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RASAL2 (RAS protein activator like 2) is a protein-coding gene that encodes a member of the RAS GTPase-activating protein (RAS-GAP) family. The encoded protein contains a characteristic GAP-related domain (GRD) that enables it to catalyze the hydrolysis of RAS-GTP to RAS-GDP, thereby functioning as a negative regulator of RAS signaling. Structurally, RASAL2 also possesses additional domains including a Pleckstrin homology (PH) domain and a C2 domain, which are involved in membrane localization and calcium/lipid binding. RASAL2 has been extensively studied for its context-dependent roles in cancer, predominantly functioning as a tumor suppressor in malignancies such as breast, bladder, renal cell, ovarian, and lung cancers, where its downregulation or loss promotes tumor growth, stemness, epithelial-mesenchymal transition (EMT), and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RASAL2 antibody 22140-1-AP | Download protocol |
| IHC protocol for RASAL2 antibody 22140-1-AP | Download protocol |
| WB protocol for RASAL2 antibody 22140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-RASAL2 antibody (Cat# 22140-1-AP) has 6 citations across journals including Int J Biol Sci, J Transl Med, Mol Metab, Transl Res, J Clin Transl Hepatol, and Mol Cell Endocrinol. Research fields span pancreatic ductal adenocarcinoma, triple-negative breast cancer, obesity and adipogenesis, hypoxic renal interstitial fibrosis, hepatic steatosis, and polycystic ovary syndrome. Applications include WB, IP, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Int J Biol Sci RASAL2 mediated the enhancement of YAP1/TIAM1 signaling promotes malignant phenotypes of pancreatic ductal adenocarcinoma
| ||
J Transl Med Rasal2 inhibits autophagic-exosomes secretion via regulating Rab27a in triple-negative breast cancer progression | ||
Mol Metab Rasal2 deficiency reduces adipogenesis and occurrence of obesity-related disorders.
| ||
Transl Res RAS protein activator-like 2 (RASAL2) initiates peritubular capillary rarefaction in hypoxic renal interstitial fibrosis | ||
J Clin Transl Hepatol RASAL2 Deficiency Attenuates Hepatic Steatosis by Promoting Hepatic VLDL Secretion via the AKT/TET1/MTTP Axis
| ||
Mol Cell Endocrinol A novel role of follicular fluid exosomal miR-143-5p in polycystic ovary syndrome: targeting RASAL2 to drive granulosa cell proliferation. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 out of 5. The user reported clean bands at the expected molecular weight of approximately 140 kDa in both human (A549) and mouse (MEF) cell lines using Western Blot at a 1:1000 dilution. Overall, the feedback indicates reliable specificity and performance for the intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
anwesh (Verified Customer) (11-14-2019) | Clean bands observed at the right size of ~140KD in both human and mouse cell lines
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