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Rabenosyn 5 Polyclonal antibody (22218-1-AP)

Rabenosyn 5 Polyclonal antibody

Rabenosyn 5 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 22218-1-AP

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

ZFYVE20, 110 kDa protein, FYVE finger-containing Rab5 effector protein rabenosyn-5, Rabenosyn-5, RBSN

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis using 22218-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of K-562 using 22218-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 22218-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse heart using 22218-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse brain using 22218-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A549 using 22218-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse skeletal muscle using 22218-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse kidney using 22218-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse heart using 22218-1-AP
IP result of anti-Rabenosyn 5 (IP:22218-1-AP, 4ug; Detection:22218-1-AP 1:10000) with mouse heart tissue lysate 1920 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 22218-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 22218-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 22218-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 22218-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for Rabenosyn 5 in ?

Overview

Tested Applications
Product Information
Publications (6)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A549 cells, K-562 cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, mouse testis tissue, Calu-1 cells, rat heart tissue
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human testis tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22218-1-AP targets Rabenosyn 5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17554 Product name: Recombinant human ZFYVE20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 437-784 aa of BC106940 Sequence: LRKAEGWLPLSGGQGQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVRTLQENLRQLQDEYDQQQTEKAIELSRRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFREIGPFQLEPSREPRTHLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHEGPSLNPFDEEDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQPDSPAPNPFSEEDEHPQQRLSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLLQQIDNIKAYIFDAKQCGRLDEVEVLTENLRELKHTLAKQKGGTD Predict reactive species
Full Name	zinc finger, FYVE domain containing 20
Calculated Molecular Weight	784 aa, 89 kDa
Observed Molecular Weight	110 kDa
GenBank Accession Number	BC106940
Gene Symbol	Rabenosyn 5
Gene ID (NCBI)	64145
RRID	AB_11182179
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H1K0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for Rabenosyn 5 antibody 22218-1-AP	Download protocol
IP protocol for Rabenosyn 5 antibody 22218-1-AP	Download protocol
WB protocol for Rabenosyn 5 antibody 22218-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The Rabenosyn 5 Polyclonal antibody (Cat# 22218-1-AP) has 6 total citations. These appear in Nature Communications, EMBO Reports, Journal of Gastroenterology, International Journal of Molecular Sciences, and Journal of Molecular Biology. The cited research spans vesicle trafficking and SNARE targeting, LDLR-mediated lipoprotein endocytosis, hepatocellular carcinoma progression via integrin recycling, Rab5 inactivation after DNA damage, endolysosomal tethering factor interactions, and metastasis suppressor identification in triple-negative breast cancer.

Species	Application	Title
human	WB,IF	Nat Commun Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic Authors - Seiichi Koike View Article
mouse	IF	EMBO Rep Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance. Authors - Ye Chen View Article
human	WB	J Gastroenterol VPS45 promotes the progression of hepatocellular carcinoma by recycling β1 integrin to the cell membrane via the endocytic pathway. Authors - Takashi Ofuchi View Article
mouse	WB,IP,IF	Int J Mol Sci Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage Authors - Masato Mashimo View Article
human	WB,IHC	J Mol Biol Identification of New Interactions between Endolysosomal Tethering Factors. Authors - Zsófia Simon-Vecsei View Article
		Nat Commun In vivo CRISPR screening identifies metastasis suppressors in triple-negative breast cancer. Authors - Soaad Galal View Article