Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart |
| Positive IHC detected in | human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22281-1-AP targets MYH6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken, zebrafish, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 6, cardiac muscle, alpha |
| Calculated Molecular Weight | 224 kDa |
| Observed Molecular Weight | 200-220 kDa |
| GenBank Accession Number | NM_002471 |
| Gene Symbol | MYH6 |
| Gene ID (NCBI) | 4624 |
| RRID | AB_2736822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13533 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH6 antibody 22281-1-AP | Download protocol |
| IHC protocol for MYH6 antibody 22281-1-AP | Download protocol |
| WB protocol for MYH6 antibody 22281-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYH6 polyclonal antibody (Cat# 22281-1-AP) has 71 citations spanning journals including Science, Cell Stem Cell, Nature Communications, Cell Death & Disease, Developmental Cell, EMBO Molecular Medicine, Cell Reports, iScience, and eLife. Research fields predominantly cover cardiac pathology (heart failure, cardiomyopathy, cardiac remodeling), skeletal muscle biology (myoblast differentiation, sarcopenia), stem cell-derived cardiomyocyte generation, and metabolic disorders such as diabetic and obese cardiomyopathy. Cited applications include WB, IF, and IHC across human, mouse, rat, and other species.
| Species | Application | Title |
|---|---|---|
Science HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy. | ||
Cell Stem Cell Epi-Allele elicits compensatory expression of the non-targeted allele and prevents haploinsufficiency in dominant genetic diseases | ||
Nat Commun Fluorofenidone ameliorates pressure overload-induced heart failure by stabilizing SERCA2a in male mice. | ||
Redox Biol iPSC-derived cardiomyocytes and engineered heart tissues reveal suppressed JAK2/STAT3 signaling in LMNA-related emery-dreifuss muscular dystrophy | ||
Theranostics Ascorbic acid induces MLC2v protein expression and promotes ventricular-like cardiomyocyte subtype in human induced pluripotent stem cells derived cardiomyocytes | ||
Smart Med Mechanistic Insights Into NFIX-Mediated DNA Recognition and Transcriptional Regulation in Skeletal Muscle. |
Reviews
What customers say
All four reviews are for Western Blot applications, with an average rating of 4.75/5. Users consistently report strong, specific signal with clean background, detecting a single band around 230–260 kDa in heart tissue, mouse heart, and iPSC-derived cardiomyocytes. One reviewer noted the band was not very sharp and suggested using a lower percentage gel for improved resolution. Tested dilutions ranged from 1:1,000 to 1:8,000. Overall, reviewers describe the antibody as reliable and well-performing for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Charlène (Verified Customer) (04-16-2025) | The antibody is working well on our samples
 |
Hua (Verified Customer) (01-20-2023) | Very good antibody. Strong signal and clean background in WB.
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Jane (Verified Customer) (02-03-2022) | one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.
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Jing (Verified Customer) (02-08-2021) | good antibody, clear sigle band around 260KD.
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