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What's the best dilution for MYH15 in ?
Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22290-1-AP targets MYH15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 15 |
| Calculated Molecular Weight | 225 kDa |
| Observed Molecular Weight | 220-240 kDa |
| GenBank Accession Number | NM_014981 |
| Gene Symbol | MYH15 |
| Gene ID (NCBI) | 22989 |
| RRID | AB_3085694 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2K3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYH15 antibody 22290-1-AP | Download protocol |
| WB protocol for MYH15 antibody 22290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYH15 Polyclonal antibody (Cat# 22290-1-AP) has 1 citation to date. It appears in Advanced Healthcare Materials (IF: 11), published in 2026. The cited study, "Spiky Nanoparticle-Gel Composite for Efficient Intravesical Botulinum Toxin A Delivery and Treatment of Overactive Bladder," used this antibody for Western blot in human samples. The research falls within the fields of drug delivery systems and urological disease treatment (overactive bladder).
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Spiky Nanoparticle-Gel Composite for Efficient Intravesical Botulinum Toxin A Delivery and Treatment of Overactive Bladder. |
