Tested Applications
| Positive WB detected in | PC-3 cells, HeLa cells, A431 cells, Jurkat cells, HL-60 cells, mouse thymus tissue |
| Positive IP detected in | THP-1 cells |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22565-1-AP targets ELF1 in WB, IHC, IF/ICC, IP, chIP, ELISA, EMSA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14689 Product name: Recombinant human ELF1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 320-618 aa of BC030507 Sequence: TSNRNQTSRSRVSSSPGVKGGATTVLKPGNSKAAKPKDPVEVAQPSEVLRTVQPTQSPYPTQLFRTVHVVQPVQAVPEGEAARTSTMQDETLNSSVQSIRTIQAPTQVPVVVSPRNQQLHTVTLQTVPLTTVIASTDPSAGTGSQKFILQAIPSSQPMTVLKENVMLQSQKAGSPPSIVLGPAQVQQVLTSNVQTICNGTVSVASSPSFSATAPVVTFSPRSSQLVAHPPGTVITSVIKTQETKTLTQEVEKKESEDHLKENTEKTEQQPQPYVMVVSSSNGFTSQVAMKQNELLEPNS Predict reactive species |
| Full Name | E74-like factor 1 (ets domain transcription factor) |
| Calculated Molecular Weight | 619 aa, 67 kDa |
| Observed Molecular Weight | 97 kDa |
| GenBank Accession Number | BC030507 |
| Gene Symbol | ELF1 |
| Gene ID (NCBI) | 1997 |
| RRID | AB_2879123 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32519 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ELF1, also named as ETS-related transcription factor Elf-1, is originally cloned from a human T-cell cDNA library by hybridization with a probe encoding the DNA binding domain (ETS domain) of the human Ets-1 cDNA. Based on its preferential expression in embryonic lymphoid organs (thymus and spleen), a wide variety of epithelial cells and fetal liver as well as in adult haematopoietic tissues, including thymus, spleen and bone marrow, Elf-1 emerged as a potential key regulator of haematopoietic gene expression. Consistent with this notion, Elf-1 has been shown to be a direct upstream regulator of genes important for haematopoiesis such as Scl, Fli-1, Lyl-1, Runx1 and Lmo2 . Elf-1 has also been shown to be important for blood vessel development, a process that is closely linked to early haematopoiesis during embryonic development. Elf-1 has been reported to take part in the transcriptional control of major regulators of blood vessel development such as Tie1, Tie2, angiopoietin-2, the vascular endothelial growth factor receptor 1 (VEGFR1), the endothelial nitric-oxide synthase (eNOS) and endoglin . Functional activity of Ets proteins is modulated at multiple levels. It is known that ELF-1 appears in the cytoplasm as a 80 KDa protein that is O -glycosylated and phosphorylated in order to be translocated into the nucleus where it can be detected as a 98 KDa protein. After dephosphorylation, the protein is degraded through the proteasome pathway. The inactive form of Elf-1 is an 80-kDa protein that lacks DNA-binding activity and is confined to the cytoplasm of the cell. Phosphorylation and O-linked glycosylation increase the molecular weight of Elf-1 to 98 kDa, the active form; 98 kDa Elf-1 binds to the promoter of the gene that codes for CD3ζ inducing its transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ELF1 antibody 22565-1-AP | Download protocol |
| IHC protocol for ELF1 antibody 22565-1-AP | Download protocol |
| IP protocol for ELF1 antibody 22565-1-AP | Download protocol |
| WB protocol for ELF1 antibody 22565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ELF1 Polyclonal antibody (Cat# 22565-1-AP) has 13 citations published in journals including Nature, Nature Genetics, Leukemia, Journal of Experimental & Clinical Cancer Research, Acta Pharmacologica Sinica, Journal of Autoimmunity, Neoplasia, BMC Cancer, and others. Research fields span transcriptional regulation, acute myeloid leukemia, prostate and colorectal cancer, ferroptosis, cardiomyocyte pyroptosis, neuroinflammation, autoimmune disease, and gastric cancer. Applications include WB, IHC, ChIP, IF, and EMSA in human and mouse models.
| Species | Application | Title |
|---|---|---|
Nat Genet Pharmacological restriction of genomic binding sites redirects PU.1 pioneer transcription factor activity | ||
J Exp Clin Cancer Res TNFAIP8 promotes AML chemoresistance by activating ERK signaling pathway through interaction with Rac1. | ||
Acta Pharmacol Sin METTL14-mediated m6A methylation of pri-miR-5099 to facilitate cardiomyocyte pyroptosis in myocardial infarction
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Leukemia G3BP2 confers venetoclax resistance by stabilizing ELF1-mediated MCL1 transcription in acute myeloid leukemia. | ||
Int J Biol Macromol ETS transcription factor 1-insulin-like growth factor 2 mRNA-binding protein 2-interferon-induced transmembrane protein 3 pathway promotes ferroptosis-driven osteoblast senescence in diabetic osteoporosis.
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Reviews
What customers say
The single review for this product was submitted in June 2020 for a Western Blot application using fallopian tube epithelial cells at a 1:1000 dilution. The reviewer rated the product 3 out of 5, noting that while the antibody detected the target, it also produced multiple non-specific bands, indicating room for improved specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Iru (Verified Customer) (06-18-2020) | There are other non-specific multiple bands as well.
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