Tested Applications
| Positive WB detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22608-1-AP targets ZZZ3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18362 Product name: Recombinant human ZZZ3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 555-903 aa of BC035079 Sequence: YPQRVVQLPEIVWDQYTHSLGNFEREFKNRKRHTRRVKLVFGKVGLPARPKSPLDPKKDGESLSYSMLPLSDGPEGSSSRPQMIRGRLCDDTKPETFNQLWTVEEQKKLEQLLIKYPPEEVESRRWQKIADELGNRTAKQVASQVQKYFIKLTKAGIPVPGRTPNLCIYSKKSSTSRRQHPLNKHLFKPSTFMTSHEPPVYMDEDDDRSCFHSHMNTAVEDASDDESIPIMYRNLPEYKELLQFKKLKKQKLQQMQAESGFVQHVGFKCDNCGIEPIQGVRWHCQDCPPEMSLDFCDSCSDCLHETDIHKEDHQLEPIYRSETFLDRDYCVSQGTSYNYLDPNYFPANR Predict reactive species |
| Full Name | zinc finger, ZZ-type containing 3 |
| Calculated Molecular Weight | 903 aa, 102 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | NM_015534.6 |
| Gene Symbol | ZZZ3 |
| Gene ID (NCBI) | 26009 |
| RRID | AB_2879134 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q8IYH5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zinc finger, ZZ-type containing 3 (ZZZ3) is a component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZZZ3 antibody 22608-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZZZ3 Polyclonal antibody (Cat# 22608-1-AP) has 1 citation. It was cited in Cardiovascular Research (IF 12.5, 2026) in a study on long noncoding RNA CARDINAL cis-activating MYOCD expression via histone reader ZZZ3 in vascular smooth muscle cell phenotype alteration. The associated research field is cardiovascular biology, specifically vascular smooth muscle cell regulation and epigenetic mechanisms.
| Species | Application | Title |
|---|---|---|
Cardiovasc Res Long noncoding RNA CARDINAL cis-activates MYOCD expression by recruiting histone reader ZZZ3 in vascular smooth muscle cell phenotype alteration. |
Reviews
What customers say
The single review for this antibody reports a 3-star rating from a Western Blot application on lymphoblastoid cells at a 1:1000 dilution. The reviewer noted the appearance of multiple bands, questioning whether they were aspecific. Overall, the feedback suggests the antibody may produce some non-specific binding under the tested conditions, though the reviewer did not provide additional details on optimization attempts or alternative results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Baptiste (Verified Customer) (08-28-2026) | Multiples bands (aspecific?)
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