Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22788-1-AP targets HIC2 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18789 Product name: Recombinant human HIC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 161-430 aa of BC094787 Sequence: RSQRLSTASVIQARYQGLVDGRKGAHAPQELPQAKGSDDELFLGGSNQDSVQGLGRAVCPAGGEAGLGGCSSSTNGSSGGCEQELGLDLSKKSPPLPPATPGPHLTPDDAAQLSDSQHGSPPAASAPPVANSASYSELGGTPDEPMDLEGAEDNHLSLLEAPGGQPRKSLRHSTRKKEWGKKEPVAGSPFERREAGPKGPCPGEEGEGVGDRVPNGILASGAGPSGPYGEPPYPCKEEEENGKDASEDSAQSGSEGGSGHASAHYMYRQE Predict reactive species |
| Full Name | hypermethylated in cancer 2 |
| Calculated Molecular Weight | 615 aa, 66 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC094787 |
| Gene Symbol | HIC2 |
| Gene ID (NCBI) | 23119 |
| RRID | AB_2879169 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JB3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumour suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for HIC2 antibody 22788-1-AP | Download protocol |
| WB protocol for HIC2 antibody 22788-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-HIC2 antibody (Cat# 22788-1-AP) has 5 total citations published in Nature Genetics, Blood, Free Radical Biology and Medicine, American Journal of Cancer Research, and bioRxiv. The research spans developmental hemoglobin switching (BCL11A regulation), glioblastoma progression via TGF-β signaling suppression, prostate cancer aggressiveness through the miR-6068/HIC2/SIRT1 axis, and fetal globin transcript regulation by RNA-binding proteins. All cited studies used human samples, with Western blot as the primary application.
| Species | Application | Title |
|---|---|---|
Nat Genet HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription
| ||
Blood Let-7 miRNAs repress HIC2 to regulate BCL11A transcription and hemoglobin switching | ||
Free Radic Biol Med HIC2 suppresses glioblastoma progression via transcriptional repression of SEMA3A and inhibition of TGF-β signaling | ||
Am J Cancer Res Small extracellular vesicle-associated miR-6068 promotes aggressive phenotypes of prostate cancer through miR-6068/HIC2/SIRT1 axis | ||
bioRxiv Direct and indirect regulation of fetal globin transcript by RNA-binding protein IGF2BP1 |
