C20orf194 Polyclonal antibody

C20orf194 Polyclonal Antibody for WB, ELISA

Cat No. 23184-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

C20orf194

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of mouse testis using 23184-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 23184-1-AP (C20orf194 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of rat testis using 23184-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 23184-1-AP (C20orf194 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 23184-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23184-1-AP (C20orf194 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 23184-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23184-1-AP (C20orf194 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 23184-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23184-1-AP (C20orf194 Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human brain using 23184-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23184-1-AP (C20orf194 Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

mouse testis tissue, rat testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23184-1-AP targets C20orf194 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19714 Product name: Recombinant human C20orf194 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 568-916 aa of BC122529 Sequence: GYTDVIDVVQALQTHPDSNVKASFTIGAITACVEPMSCYMEHRFLFPKCLDQCSQGLVSNVVFTSHTTEQRHPLLVQLQSLIRAANPAAAFILAENGIVTRNEDIELILSENSFSSPEMLRSRYLMYPGWYEGKLNAGSVYPLMVQICVWFGRPLEKTRFVAKCKAIQSSIKPSPFSGNIYHILGKVKFSDSERTMEVCYNTLANSLSIMPVLEGPTPPPDSKSVSQDSSGQQECYLVFIGCSLKEDSIKDWLRQSAKQKPQRKALKTRGMLTQQEIRSIHVKRHLEPLPAGYFYNGTQFVNFFGDKTDFHPLMDQFMNDYVEEANREIEKYNQELEQQEYHDLFELKP Predict reactive species
Full Name	chromosome 20 open reading frame 194
Calculated Molecular Weight	1177 aa, 132 kDa
Observed Molecular Weight	132 kDa
GenBank Accession Number	BC122529
Gene Symbol	C20orf194
Gene ID (NCBI)	25943
RRID	AB_2879227
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q5TEA3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for C20orf194 antibody 23184-1-AP	Download protocol
WB protocol for C20orf194 antibody 23184-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The C20orf194 Polyclonal antibody (Cat# 23184-1-AP) has 1 citation to date. It appears in Nature Structural & Molecular Biology (2025), in a study investigating the molecular basis for activation of outer dynein arms in cilia. The research field is cilia biology and structural/molecular biology. The antibody was used for Western blot and immunofluorescence applications.