Tested Applications
| Positive WB detected in | rat skeletal muscle tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23880-1-AP targets MYOZ3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20839 Product name: Recombinant human MYOZ3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-251 aa of BC074870 Sequence: MIPKEQKGPVMAAMGDLTEPVPTLDLGKKLSVPQDLMMEELSLRNNRGSLLFQKRQRRVQKFTFELAASQRAMLAGSARRKVTGTAESGTVANANGPEGPNYRSELHIFPASPGASLGGPEGAHPAAAPAGCVPSPSALAPGYAEPLKGVPPEKFNHTAIPKGYRCPWQEFVSYRDYQSDGRSHTPSPNDYRNFNKTPVPFGGPLVGGTFPRPGTPFIPEPLSGLELLRLRPSFNRVAQGWVRNLPESEEL Predict reactive species |
| Full Name | myozenin 3 |
| Calculated Molecular Weight | 251 aa, 27 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC074870 |
| Gene Symbol | MYOZ3 |
| Gene ID (NCBI) | 91977 |
| RRID | AB_2879347 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q8TDC0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYOZ3 antibody 23880-1-AP | Download protocol |
| WB protocol for MYOZ3 antibody 23880-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The antibody received a 5-star rating for use in Western Blot and Immunofluorescence on murine skeletal muscle. The reviewer shared detailed protocols: for IF on fixed cryosections, they used 0.3% Triton permeabilization, blocked with 0.5% BSA and 10% goat serum, then incubated with primary antibody at 1:100 overnight at 4°C and secondary at 1:200 for 2 hours at room temperature. For WB, they blocked with 5% milk in TBS-T, used primary at 1:1000 overnight at 4°C and secondary at 1:2000 for 2 hours, yielding clear double bands between 39 and 28 kDa. Overall, the product performed well with strong, specific results.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Martina (Verified Customer) (07-03-2023) | IF protocol for fixed cryosection of mouse skeletal muscle: after a quick permeabilization step with Triton 0,3% in PBS, block with BSA 0,5% and GS 10% in PBS for at least 2 hours at room temperature. Then incubate with primary antibody 1:100 in blocking solution overnight at +4°C. The day after, incubate with secondary antibody 1:200 in blocking solution for 2 hours at room temperature. WB protocol for mouse skeletal muscle proteins: block the membrane with 5% milk in TBS-T for at least 2 hours at room temperature; then incubate with primary antibody 1:1000 in 5% milk in TBS-T at +4°C overnight. The day after, incubate with secondary antibody 1:2000 in 5% milk in TBS-T for 2 hours at room temperature. Developing the membrane, you will see double bands between 39 and 28 kDa.
 |
