Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23937-1-AP targets ACIN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21036 Product name: Recombinant human ACIN1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 979-1328 aa of BC140805 Sequence: TIDDPVRTAQVPSPPRGKISNIVHISNLVRPFTLGQLKELLGRTGTLVEEAFWIDKIKSHCFVTYSTVEEAVATRTALHGVKWPQSNPKFLCADYAEQDELDYHRGLLVDRPSETKTEEQGIPRPLHPPPPPPVQPPQHPRAEQREQERAVREQWAEREREMERRERTRSEREWDRDKVREGPRSRSRSRDRRRKERAKSKEKKSEKKEKAQEEPPAKLLDDLFRKTKAAPCIYWLPLTDSQIVQKEAERAERAKEREKRRKEQEEEEQKEREKEAERERNRQLEREKRREHSRERDRERERERERDRGDRDRDRERDRERGRERDRRDTKRHSRSRSRSTPVRDRGGRR Predict reactive species |
| Full Name | apoptotic chromatin condensation inducer 1 |
| Calculated Molecular Weight | 1341 aa, 152 kDa |
| Observed Molecular Weight | 250 kDa, 80-85 kDa |
| GenBank Accession Number | BC140805 |
| Gene Symbol | ACIN1 |
| Gene ID (NCBI) | 22985 |
| RRID | AB_3085716 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKV3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Apoptosis is defined by several morphologic nuclear changes, including chromatin condensation and nuclear fragmentation. ACIN1 is a nuclear protein that induces apoptotic chromatin condensation after activation by caspase-3, without inducing DNA fragmentation. This protein has also been shown to be a component of a splicing-dependent multiprotein exon junction complex (EJC) that is deposited at splice junctions on mRNAs, as a consequence of pre-mRNA splicing. There are three isoforms of human acinus (the L, S and S' forms) shows at about 80-85 kDa and 220-250 kDa by Western Blot, which were probably generated by alternative splicing (PMID: 10490026). 23937-1-AP can detect all three isoforms of ACIN1. Another antibody 30522-1-AP specificly targets at the ACIN1-L.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ACIN1 antibody 23937-1-AP | Download protocol |
| WB protocol for ACIN1 antibody 23937-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACIN1 Polyclonal antibody (Cat# 23937-1-AP) has 1 total citation. It appears in Scientific Reports (IF 4.9), published in 2025. The cited study investigates the impact of PANoptosis-related genes on immune profiles and subtype classification in ischemic stroke, using this antibody for Western Blot in mouse samples. The associated research fields include stroke pathology, PANoptosis, and immune profiling.
| Species | Application | Title |
|---|---|---|
Sci Rep The impact of PANoptosis-related genes on immune profiles and subtype classification in ischemic stroke. |
