Tested Applications
| Positive WB detected in | HL-60 cells, human brain tissue, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23945-1-AP targets IGHMBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21057 Product name: Recombinant human IGHMBP2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 642-993 aa of BC105088 Sequence: YLDDIVPENYSHENSQGSSHAATKPQGPAASTRTGSQRQEGGQEAAAPARQGRKKPAGKSLASEAPSQPSLNGGSPEGVESQDGVDHFRAMIVEFMASKKMQLEFPPSLNSHDRLRVHQIAEEHGLRHDSSGEGKRRFITVSKRAPRPRAALGPPAGTGGPAPLQPVPPTPAQTEQPPREQRGPDQPDLRTLHLERLQRVRSAQGQPASKEQQASGQQKLPEKKKKKAKGHPATDLPTEEDFEALVSAAVKADNTCGFAKCTAGVTTLGQFCQLCSRRYCLSHHLPEIHGCGERARAHARQRISREGVLYAGSGTKNGSLDPAKRAQLQRRLDKKLSELSNQRTSRRKERGT Predict reactive species |
| Full Name | immunoglobulin mu binding protein 2 |
| Calculated Molecular Weight | 993 aa, 109 kDa |
| Observed Molecular Weight | 109 kDa |
| GenBank Accession Number | BC105088 |
| Gene Symbol | IGHMBP2 |
| Gene ID (NCBI) | 3508 |
| RRID | AB_2879371 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P38935 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IGHMBP2 also named as immunoglobulin mu binding protein 2 is a 993 amino-acid protein, which localizes in nuclear and cytoplasm. The molecular weight of IGHMBP2 is 109 kDa. IGHMBP2 belongs to the DNA2/NAM7 helicase family. IGHMBP2 also acts as a transcriptional regulator and is necessary for transcriptional activation of the flounder liver-type antifreeze protein gene. IGHMBP2 exists as a homooligomer and is part of the cytosolic ribonucleoprotein complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IGHMBP2 antibody 23945-1-AP | Download protocol |
| WB protocol for IGHMBP2 antibody 23945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IGHMBP2 Polyclonal antibody (Cat# 23945-1-AP) has a total of 2 citations. Both appear in Life Science Alliance and bioRxiv, and both studies investigate how IGHMBP2 deletion suppresses translation and activates the integrated stress response. The associated research fields include molecular biology, translation regulation, and cellular stress signaling.
| Species | Application | Title |
|---|---|---|
Life Sci Alliance IGHMBP2 deletion suppresses translation and activates the integrated stress response | ||
bioRxiv IGHMBP2 deletion suppresses translation and activates the integrated stress response |
