Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human lymphoma tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23959-1-AP targets BCL3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21040 Product name: Recombinant human BCL3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 97-446 aa of BC064993 Sequence: NLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS Predict reactive species |
| Full Name | B-cell CLL/lymphoma 3 |
| Calculated Molecular Weight | 446 aa, 47 kDa |
| Observed Molecular Weight | 48-60 kDa |
| GenBank Accession Number | BC064993 |
| Gene Symbol | BCL3 |
| Gene ID (NCBI) | 602 |
| RRID | AB_2879375 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20749 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCL3 (B-cell lymphoma 3 protein) has seven ankyrin repeat domains, a proline-rich N terminal domain and a serine- and proline-rich C-terminal domain(PMID: 31930327). BCL3 is an important contributor to chronic inflammatory disease states, such as osteoarthritis and rheumatoid arthritis (PMID: 12483727). BCL3 is a predominantly nuclear protein containing a trans-activation domain, and it can be recruited to NF-κB-responsive promoters, resulting in transcriptional activation or repression depending on the subunit composition of NF-κB complexes (PMID: 25089799, PMID: 30135206). BCL3 is known to be overexpressed in human breast cancers and is associated with reduced metastasis-free survival (PMID: 38255248). The predicted molecular weight of the BCL3 protein is 48 kDa. However, due to post-translational modifications (e.g., phosphorylation), its apparent molecular weight often ranges between 55 and 65 kDa (PMID: 9407099, PMID: 12847243).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BCL3 antibody 23959-1-AP | Download protocol |
| IHC protocol for BCL3 antibody 23959-1-AP | Download protocol |
| IP protocol for BCL3 antibody 23959-1-AP | Download protocol |
| WB protocol for BCL3 antibody 23959-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BCL3 antibody (Cat# 23959-1-AP) has 17 citations spanning journals including Nature, Nucleic Acids Research, Haematologica, J Biol Chem, J Immunol, Mol Cancer Ther, iScience, and Carcinogenesis. Research fields cover oncology (colorectal, ovarian, esophageal, and renal cancers), NF-κB signaling, immune checkpoint/PD-L1 regulation, liver injury, type 1 diabetes, and neurology (astrocyte reactivity, intracerebral hemorrhage). Applications include WB, IHC, IF, IP, CoIP, and ChIP in human and mouse models.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res SPaRTAN, a computational framework for linking cell-surface receptors to transcriptional regulators. | ||
Haematologica BCL3 rearrangements in B-cell lymphoid neoplasms occur in two breakpoint clusters associated with different diseases | ||
Cell Mol Gastroenterol Hepatol Liver-specific Bcl3 knockout alleviates acetaminophen-induced liver injury by activating Nrf2 pathway in male mice | ||
Int J Oncol BCL‑3 promotes cyclooxygenase‑2/prostaglandin E2 signalling in colorectal cancer. | ||
Reviews
What customers say
Both reviewers rated this antibody 5 stars. One user found it effective for Western Blot (1:2000) and Immunofluorescence (1:200) on bone marrow MSCs. Another user, working with human lymphoma cell lines (WT and KO), reported it was the only antibody that successfully detected endogenous BCL3 by Western Blot at 1:1000, describing the performance as perfect. Overall, users highlight reliable specificity for endogenous BCL3 detection across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Udesh (Verified Customer) (08-16-2023) | Worked well for WB and IF
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Ewelina (Verified Customer) (07-03-2019) | This is the only antibody for Western Blot, that worked for me for the detection of endogenous BCL3 in human lymphoma cell lines. It works perfectly.
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