HOMEZ Polyclonal antibody

HOMEZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23965-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HOMEZ, KIAA1443

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIHCIF/ICC

WB analysis using 23965-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23965-1-AP (HOMEZ antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human testis using 23965-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 23965-1-AP (HOMEZ Antibody) at dilution of 1:50.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HEK-293 using 23965-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using HOMEZ antibody (23965-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23965-1-AP targets HOMEZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21090 Product name: Recombinant human HOMEZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC130392 Sequence: MPPNKEASGLSSSPAGLICLPPISEELQLVWTQAAQTSELDSNEHLLKTFSYFPYPSLADIALLCLRYGLQMEKVKTWFMAQRLRCGISWSSEEIEETRARVVYRRDQLHFKSLLSFTHHAGRPPEEVPPPPVPAPEQVGIGIGPPTLSKPTQTKGLKVEPEEPSQMPPLPQSHQKLKESLMTPGSGAFPYQSDFWQHLQSSGLSKEQAGRGPNQSHGIGTASWNHSTTVPQPQARDKPPPIALIASSCKEESASSVTPSSSSTSSSFQVLANGATATSKPLQPLGCVPQSVSPSEQALPPHLEPAWPQGLRHNSVPGRVGPTEYLSPDMQRQRKTKRKTKEQLAILKS Predict reactive species
Full Name	homeobox and leucine zipper encoding
Calculated Molecular Weight	525 aa, 59 kDa
Observed Molecular Weight	65 kDa
GenBank Accession Number	BC130392
Gene Symbol	HOMEZ
Gene ID (NCBI)	57594
RRID	AB_2879378
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q8IX15
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for HOMEZ antibody 23965-1-AP	Download protocol
IHC protocol for HOMEZ antibody 23965-1-AP	Download protocol
WB protocol for HOMEZ antibody 23965-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The HOMEZ Polyclonal antibody (Cat# 23965-1-AP) has 1 citation. It was used in Western Blot to study HOMEZ in mouse models, published in Translational Andrology and Urology (2022). The research field is reproductive biology, specifically investigating the role of the homeodomain protein HOMEZ in male fertility.