Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, Jurkat cells, L02 cells, mouse brain tissue, mouse liver tissue, RAW 264.7 cells, rat liver tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23987-1-AP targets ZBTB20 in WB, IHC, IF-P, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21140 Product name: Recombinant human ZBTB20 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 313-668 aa of BC029041 Sequence: EPDSVEQQFGPGAARDSQAEPTQPEQAAEAPAEGGPQTNQLETGASSPERSNEVEMDSTVITVSNSSDKSVLQQPSVNTSIGQPLPSTQLYLRQTETLTSNLRMPLTLTSNTQVIGTAGNTYLPALFTTQPAGSGPKPFLFSLPQPLAGQQTQFVTVFQPGLSTFTAQLPAPQPLASSAGHSTASGQGEKKPYECTLCNKTFTAKQNYVKHMFVHTGEKPHQCSICWRSFSLKDYLIKHMVTHTGVRAYQCSICNKRFTQKSSLNVHMRLHRGEKSYECYICKKKFSHKTLLERHVALHSASNGTPPAGTPPGARAGPPGVVACTEGTTYVCSVCPAKFDQIEQFNDHMRMHVSDG Predict reactive species |
| Full Name | zinc finger and BTB domain containing 20 |
| Calculated Molecular Weight | 668 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC029041 |
| Gene Symbol | ZBTB20 |
| Gene ID (NCBI) | 26137 |
| RRID | AB_2879389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9HC78 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZBTB20 (zinc finger and BTB domain containing 20), also known as HOF, DPZF, ODA-8S or ZNF288, is a 741 amino acid protein that localizes to the nucleus and contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed in thymus, spleen, lymph node and fetal liver, ZBTB20 exists as either a monomer or a homodimer that is thought to function as a transcription factor, playing a role in hematopoiesis, oncogenesis and immune responses. Multiple isoforms of ZBTB20 exist due to alternative splicing events.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZBTB20 antibody 23987-1-AP | Download protocol |
| IHC protocol for ZBTB20 antibody 23987-1-AP | Download protocol |
| WB protocol for ZBTB20 antibody 23987-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ZBTB20 antibody (Cat# 23987-1-AP) has 12 total citations. Journals include Bone Research, EBioMedicine, Journal of Translational Medicine, Cell Reports, Cell Proliferation, Molecular & Cellular Proteomics, International Journal of Molecular Sciences, Journal of Inflammation Research, Brain Research, Transplant Immunology, and Wellcome Open Research. Research fields span osteoarthritis, neurogenesis, lung cancer, cardiomyocyte biology, brain organoids, chromatin regulation, uveal melanoma, epigenetics, schizophrenia, cardiac transplant rejection, and Alzheimer's disease.
| Species | Application | Title |
|---|---|---|
Bone Res LATS1-modulated ZBTB20 perturbing cartilage matrix homeostasis contributes to early-stage osteoarthritis | ||
EBioMedicine Establishing the role of ZBTB20 mutations in GnRH deficiency and impaired neurogenesis in the subventricular zone: a human cohort and animal model study. | ||
J Transl Med Network-based single-cell analysis identifies a proliferative GZMB(+) plasma cell subset linked to Hedgehog signaling and prognostic risk in lung squamous cell carcinoma. | ||
Cell Rep Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes. | ||
Cell Prolif In vivo development and single-cell transcriptome profiling of human brain organoids. | ||
Mol Cell Proteomics Selective modulation of the human glucocorticoid receptor compromises GR chromatin occupancy and recruitment of p300/CBP and the Mediator complex
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