Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24312-1-AP targets CAMK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19449 Product name: Recombinant human CAMK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 319-370 aa of BC106755 Sequence: LQLGTSQEGQGQTASHGELLTPVAGGPAAGCCCRDCCVEPGTELSPTLPHQL Predict reactive species |
| Full Name | calcium/calmodulin-dependent protein kinase I |
| Calculated Molecular Weight | 370 aa, 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC106755 |
| Gene Symbol | CAMK1 |
| Gene ID (NCBI) | 8536 |
| RRID | AB_2879489 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14012 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CAMK1 antibody 24312-1-AP | Download protocol |
| IHC protocol for CAMK1 antibody 24312-1-AP | Download protocol |
| IP protocol for CAMK1 antibody 24312-1-AP | Download protocol |
| WB protocol for CAMK1 antibody 24312-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CAMK1 Polyclonal antibody (Cat# 24312-1-AP) has 5 citations published in Int J Biol Macromol, J Ethnopharmacol, Biochim Biophys Acta Mol Basis Dis, Biomed Res Int, and Clin Transl Med. Research fields span glioblastoma progression, septic cardiomyopathy and mitophagy, diabetic nephropathy (mitochondrial dynamics and m5C modification), and endothelial cell biology. Applications cited include WB and IHC in human and mouse models.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Translational hub ribosomal protein S5 promotes glioblastoma progression by affecting translation patterns. | ||
J Ethnopharmacol Qiang-Xin 1 formula improves cardiac function and alleviates myocardial injury in lipopolysaccharide-induced septic mice by activating calcium/calmodulin-dependent protein kinase I-mediated mitophagy. | ||
Biochim Biophys Acta Mol Basis Dis IGF2BP3-stabilized CAMK1 regulates the mitochondrial dynamics of renal tubule to alleviate diabetic nephropathy | ||
Biomed Res Int Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells. | ||
Clin Transl Med NSUN4-dependent m5C modification facilitates mitochondrial fission to accelerate diabetic nephropathy process via regulating SMURF1/CAMK1 axis. |
