Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, PC-3 cells |
| Positive IHC detected in | mouse adrenal gland tissue, rat adrenal gland tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24345-1-AP targets UHRF1BP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19677 Product name: Recombinant human UHRF1BP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1053-1400 aa of BC137120 Sequence: LGRDRMTSTMHKMLSLPPAKEPMAKTDEGVAAPVSGGAARLRFFSMKRTVSQQSFDGVSLDSSGPEDRISVDSDGSDSFVMLLESESGPESVPPGSLSNVSDNAGVQGSPLVNNYGQGSPAANSSVSPSGEDLIFHPVSVLVLKVNEVSFGIEVRGEDLTVALQAEELTLQQLGTVGLWQFLHGQCPGTCFQESSTLKTGHIRPAVGLRFEVGPGAAVHSPLASQNGFLHLLLHGCDLELLTSVLSGLGPFLEDEEIPVVVPMQIELLNSSITLKDDIPPIYPTSPGPIPITLAMEHVVLKRSDDGVFHIGAAAQDKPSAEVLKSEKRQPPKEQVFLVPTGEVFEQQT Predict reactive species |
| Full Name | UHRF1 binding protein 1 |
| Calculated Molecular Weight | 1440 aa, 159 kDa |
| Observed Molecular Weight | 160-170 kDa |
| GenBank Accession Number | BC137120 |
| Gene Symbol | UHRF1BP1 |
| Gene ID (NCBI) | 54887 |
| RRID | AB_3742108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6BDS2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Bridge-like lipid transfer protein family member 3A (BLTP3A), also known as UHRF1BP1, is a conserved protein characterized by six repeating beta-groove modules that form a hydrophobic groove for bulk lipid transfer between membranes. It localizes to VAMP7-positive vesicles and anchors them to LAMP1-positive lysosomes via its chorein domain and Rab7 interaction. Upon lysosomal membrane damage, BLTP3A rapidly dissociates and is re-recruited through its LIR motif binding to mATG8 proteins via the CASM pathway. This dynamic behavior suggests BLTP3A delivers lipids to aid in lysosomal repair (PMID: 40935891). Beyond its cellular function, BLTP3A has been identified as a genetic risk factor for systemic lupus erythematosus.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UHRF1BP1 antibody 24345-1-AP | Download protocol |
| WB protocol for UHRF1BP1 antibody 24345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UHRF1BP1 Polyclonal antibody (Cat# 24345-1-AP) has 1 total citation. The citation appears in bioRxiv, a preprint server. The associated research field is Parkinson's Disease, specifically investigating how WDR44 drives de novo α-synuclein aggregation at the lysosomal membrane and promotes neuronal dysfunction. The antibody was used in Western Blot (WB) with human samples.
| Species | Application | Title |
|---|---|---|
bioRxiv WDR44 drives de novo α-synuclein aggregation at the lysosomal membrane and promotes neuronal dysfunction in Parkinson's Disease.
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