Tested Applications
| Positive WB detected in | DU 145 cells, PC-3 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24408-1-AP targets MICALL2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19931 Product name: Recombinant human MICALL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 306-605 aa of BC037988 Sequence: PAATSATSVHVRSPARPSESRLAPTPTEGKVRPRVTNSSPMGWSSAAPCTAAAASHPAVPPSAPDPRPATPQGGGAPRVAAPQTTLSSSSTSAATVDPPAWTPSASRTQQARNKFFQTSAVPPGTSLSGRGPTPSLVLSKDSSKEQARNFLKQALSALEEAGAPAPGRPSPATAAVPSSQPKTEAPQASPLAKPLQSSSPRVLGLPSRMEPPAPLSTSSTSQASALPPAGRRNLAESSGVGRVGAGSRPKPEAPMAKGKSTTLTQDMSTSLQEGQEDGPAGWRANLKPVDRRSPAERTLK Predict reactive species |
| Full Name | MICAL-like 2 |
| Calculated Molecular Weight | 904 aa, 98 kDa |
| Observed Molecular Weight | 109 kDa |
| GenBank Accession Number | BC037988 |
| Gene Symbol | MICALL2 |
| Gene ID (NCBI) | 79778 |
| RRID | AB_2879527 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IY33 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MICALL2 antibody 24408-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MICALL2 antibody (catalog number 24408-1-AP) has 9 total citations. These appear in journals including Cell Communication & Signaling, Journal of Translational Medicine, Biochemical Pharmacology, International Journal of Molecular Sciences, International Dental Journal, Biochimica et Biophysica Acta (Molecular Basis of Disease), Scientific Reports, and Archives of Biochemistry and Biophysics. The associated research fields are predominantly oncology—colorectal, renal, breast, ovarian, and head-and-neck cancers—covering tumor progression, angiogenesis, and drug resistance, plus epithelial-mesenchymal transition and pulmonary fibrosis.
| Species | Application | Title |
|---|---|---|
Cell Commun Signal MICALL2 as a substrate of ubiquitinase TRIM21 regulates tumorigenesis of colorectal cancer
| ||
J Transl Med DUSP4-mediated dephosphorylation of PSMD14 enhances PSMD14-dependent deubiquitination of MICALL2 and promotes malignant progression in clear cell renal cell carcinoma. | ||
Biochem Pharmacol MICALL2 promotes angiogenesis of colorectal cancer by regulating the EGFR/PI3K/AKT/KLF5/VEGFA axis. | ||
Biochem Pharmacol MICAL-L2, as an estrogen-responsive gene, is involved in ER-positive breast cancer cell progression and tamoxifen sensitivity via the AKT/mTOR pathway
| ||
Int J Mol Sci Comprehensive Analysis of MICALL2 Reveals Its Potential Roles in EGFR Stabilization and Ovarian Cancer Cell Invasion
| ||
Int Dent J The Role of SARS1 in Head and Neck Squamous Cell Carcinoma and its Potential as a Diagnostic and Prognostic Biomarker |
Reviews
What customers say
The single review for 24408-1-AP reports a Western Blot result at a 1:1,000 dilution using MDCK GII lysate. The reviewer notes that the blot required a long exposure of over 100 seconds and shows multiple background bands. The molecular weight marker used was BioRad Precision Plus. Overall, the review reflects a mixed experience, with the antibody producing a detectable signal but requiring extended exposure and showing some background, suggesting room for optimization in sensitivity and specificity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joleen (Verified Customer) (05-04-2019) | WB required long exposure >100s. Multiple background bands. Molecular weight is BioRad Precision Plus.
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