Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24457-1-AP targets CEP44 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19961 Product name: Recombinant human KIAA1712 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC038667 Sequence: MATGDLKRSLRNLEQVLRLLNYPEEVDCVGLIKGDPAASLPIISYSFTSYSPYVTELIMESNVELIAKNDLRFIDAVYKLLRDQFNYKPILTKKQFIQCGFAEWKIQIVCDILNCVMKKHKELSSLQKIPSQQRKKISSGKSEPPLGNEKISAEAVGVDISGRFMTSGKKKAVVIRHLYNEDNVDISEDTLSPITDVNEAVDVSDLNATEIKMPEVKVPEIKAEQQDVNVNPEITALQTMLAECQENLKKLTSIEKRLDCLEQKMKGKVMVDENTWTNLLSRVTLLETEMLLSKKNDEFIEFNEVSEDYASCSDMDLLNPHRKSEVERPASIPLSSGYSTASSDSTPRAS Predict reactive species |
| Full Name | KIAA1712 |
| Calculated Molecular Weight | 390 aa, 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC038667 |
| Gene Symbol | CEP44 |
| Gene ID (NCBI) | 80817 |
| RRID | AB_2879557 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C0F1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CEP44, also named as KIAA1712 and PS1TP3, localizes to centrosomes, spindle poles, and weakly in midbody. It is a 44 kDa centrosome protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEP44 antibody 24457-1-AP | Download protocol |
| IHC protocol for CEP44 antibody 24457-1-AP | Download protocol |
| IP protocol for CEP44 antibody 24457-1-AP | Download protocol |
| WB protocol for CEP44 antibody 24457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CEP44 Polyclonal antibody (Cat# 24457-1-AP) has 12 citations published in journals including Cell, Nature Communications, Cell Reports, Journal of Cell Biology, Journal of Cell Science, Molecular Biology of the Cell, Cytoskeleton, MicroPubl Biology, and eLife. Research fields span centriole assembly and duplication, centrosome cohesion, ciliogenesis, early embryo gene expression, O-GlcNAcylation regulation, and sperm centriole architecture, primarily in human and mouse systems.
| Species | Application | Title |
|---|---|---|
Cell Time-series reconstruction of the molecular architecture of human centriole assembly | ||
Cell Rep 3D nanoscale reconstruction of human airway multiciliogenesis reveals cellular architecture remodeling and cilia-mitochondria link via rootlets. | ||
J Cell Biol Centrosomal organization of Cep152 provides flexibility in Plk4 and procentriole positioning |
