Tested Applications
| Positive WB detected in | PC-12 cells, HepG2 cells, mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24523-1-AP targets NEU2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17386 Product name: Recombinant human NEU2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 66-380 aa of BC107053 Sequence: VQWQAQEVVAQARLDGHRSMNPCPLYDAQTGTLFLFFIAIPGQVTEQQQLQTRANVTRLCQVTSTDHGRTWSSPRDLTDAAIGPAYREWSTFAVGPGHCLQLNDRARSLVVPAYAYRKLHPIQRPIPSAFCFLSHDHGRTWARGHFVAQDTLECQVAEVETGEQRVVTLNARSHLRARVQAQSTNDGLDFQESQLVKKLVEPPPQGCQGSVISFPSPRSGPGSPAQWLLYTHPTHSWQRADLGAYLNPRPPAPEAWSEPVLLAKGSCAYSDLQSMGTGPDGSPLFGCLYEANDYEEIVFLMFTLKQAFPAEYLPQ Predict reactive species |
| Full Name | sialidase 2 (cytosolic sialidase) |
| Calculated Molecular Weight | 380 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC107053 |
| Gene Symbol | NEU2 |
| Gene ID (NCBI) | 4759 |
| RRID | AB_2879588 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3R4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Publications
What published studies show
The NEU2 Polyclonal antibody (Cat# 24523-1-AP) has 2 citations. One appears in PLoS Biology (2025), studying how the mTORC2 subunit RICTOR drives breast cancer progression via ganglioside biosynthesis, using WB in human samples. The other is in Experimental Lung Research (2022), examining changes in lung sialidases in mice after bleomycin aspiration, using IF. The associated research fields are oncology/signaling and pulmonary pathology.
| Species | Application | Title |
|---|---|---|
PLoS Biol The mTORC2 subunit RICTOR drives breast cancer progression by promoting ganglioside biosynthesis through transcriptional and epigenetic mechanisms. | ||
Exp Lung Res Changes in lung sialidases in male and female mice after bleomycin aspiration |
