Tested Applications
| Positive WB detected in | HeLa cells, mouse kidney tissue, HepG2 cells, rat kidney tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24524-1-AP targets RNF145 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21463 Product name: Recombinant human RNF145 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 492-663 aa of BC042684 Sequence: IHSYYNVWLRAQLGWKSFLLRRDAVNKIKSLPIATKEQLEKHNDICAICYQDMKSAVITPCSHFFHAGCLKKWLYVQETCPLCHCHLKNSSQLPGLGTEPVLQPHAGAEQNVMFQEGTEPPGQEHTPGTRIQEGSRDNNEYIARRPDNQEGAFDPKEYPHSAKDEVHPVESA Predict reactive species |
| Full Name | ring finger protein 145 |
| Calculated Molecular Weight | 663 aa, 76 kDa |
| Observed Molecular Weight | 76-79 kDa |
| GenBank Accession Number | BC042684 |
| Gene Symbol | RNF145 |
| Gene ID (NCBI) | 153830 |
| RRID | AB_2879589 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RNF145 antibody 24524-1-AP | Download protocol |
| WB protocol for RNF145 antibody 24524-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RNF145 Polyclonal antibody (Cat# 24524-1-AP) has 5 total citations. These appear in EMBO Journal, Journal of Agricultural and Food Chemistry, Oncology Research, World Journal of Surgical Oncology, and eLife. Research fields span lipid metabolism and membrane fluidity regulation, mTOR signaling and milk synthesis in mammary epithelial cells, hepatocellular carcinoma metastasis and prognosis, and sterol-responsive ubiquitin-mediated degradation of HMG-CoA reductase. Applications include Western blotting and immunoprecipitation, primarily in human and mouse samples.
| Species | Application | Title |
|---|---|---|
EMBO J Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2
| ||
J Agric Food Chem Methionine and Leucine Promote mTOR Gene Transcription and Milk Synthesis in Mammary Epithelial Cells through the eEF1Bα-UBR5-ARID1A Signaling | ||
Oncol Res RNF145 Promotes Hepatocellular Carcinoma Metastasis through Ubiquitination and Degradation of PCDH9.
| ||
World J Surg Oncol Hypoxia-based classification and prognostic signature for clinical management of hepatocellular carcinoma | ||
Elife The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. |
