Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human cervical cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24542-1-AP targets RPRD1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21578 Product name: Recombinant human RPRD1B protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 79-181 aa of BC033629 Sequence: REFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELI Predict reactive species |
| Full Name | regulation of nuclear pre-mRNA domain containing 1B |
| Calculated Molecular Weight | 326 aa, 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC033629 |
| Gene Symbol | RPRD1B |
| Gene ID (NCBI) | 58490 |
| RRID | AB_2879597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPRD1B, also known as CREPT, is a protein-coding gene located on chromosome 20q11.23 that encodes a 326-amino-acid nuclear protein. RPRD1B functions primarily as a scaffold for the C-terminal domain (CTD) of RNA polymerase II (RNAPII), interacting with phosphorylated CTD repeats via its CTD-interaction domain (CID) and coordinating the dephosphorylation of phospho-Ser5 by the RPAP2 phosphatase. It forms homodimers and heterodimers with its paralog RPRD1A through coiled-coil domains and participates in transcription-coupled RNA processing and RNAPII promoter clearance. Functionally, RPRD1B enhances the expression of cell cycle-related genes, notably cyclin D1 (CCND1), by promoting RNAPII binding to the CCND1 promoter and facilitating loop formation that may enable RNAPII recycling during transcription. Beyond its physiological roles in transcription regulation and cell proliferation, RPRD1B is frequently overexpressed across multiple cancer types and has been shown to promote tumorigenesis by accelerating cell cycle progression, enhancing proliferative signaling, and supporting cancer cell migration and invasion, positioning it as a promising diagnostic and prognostic biomarker.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPRD1B antibody 24542-1-AP | Download protocol |
| IHC protocol for RPRD1B antibody 24542-1-AP | Download protocol |
| WB protocol for RPRD1B antibody 24542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPRD1B Polyclonal antibody (Cat# 24542-1-AP) has 3 total citations. These appear in J Diabetes Res, Discover Oncology, and bioRxiv. The research fields span diabetic nephropathy (polyamine metabolism biomarkers), pan-cancer analysis (diagnostic and prognostic biomarker evaluation), and mitochondrial biogenesis defects in PINK1-deficient human dopamine neurons. All cited studies used the antibody in human samples via WB or IHC.
| Species | Application | Title |
|---|---|---|
J Diabetes Res Identification and Verification of Biomarkers Related to Polyamine Metabolism in Diabetic Nephropathy. | ||
Discov Oncol Comprehensive pan-cancer analysis of RPRD1B as a promising diagnostic and prognostic biomarker.
| ||
bioRxiv Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PINK1-deficient Human Dopamine Neurons
|
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used it for Western Blot at a 1:1000 dilution on Jurkat nuclear cell extracts and reported it worked well, producing a major band between 30 and 42 KDa. Overall, the feedback is positive, confirming reliable performance and clear band detection in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Fabienne (Verified Customer) (05-09-2025) | Works well in western blot (major band between 30 and 42 KDa). Tested on nuclear cell extracts
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