Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24543-1-AP targets ZNF746 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21566 Product name: Recombinant human ZNF746 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 186-531 aa of BC068505 Sequence: MQIKQEGELQLQEQQALGVEAWAAGQPDIGEEPWGLSQLDSGAGDISTDATSGVHSNFSTTIPPTSWQTDLPPHHPSSACSDGTLKLNTAASTEDVKIVIKTEVQEEEVVATPVHPTDLEAHGTLFGPGQATRFFPSPAQEGAWESQGSSFPSQDPVLGLREPARPERDMGELSPAVAQEETPPGDWLFGGVRWGWNFRCKPPVGLNPRTGPEGLPYSSPDNGEAILDPSQAPRPFNEPCKYPGRTKGFGHKPGLKKHPAAPPGGRPFTCATCGKSFQLQVSLSAHQRSCGAPDGSGPGTGGGGSGSGGGGGGSGGGSARDGSALRCGECGRCFTRPAHLIRHRML Predict reactive species |
| Full Name | zinc finger protein 746 |
| Calculated Molecular Weight | 644 aa, 69 kDa |
| Observed Molecular Weight | 85-95 kDa |
| GenBank Accession Number | BC068505 |
| Gene Symbol | ZNF746 |
| Gene ID (NCBI) | 155061 |
| RRID | AB_2879598 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6NUN9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN (PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF746 antibody 24543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-ZNF746 (PARIS) antibody (Cat# 24543-1-AP) has 12 total citations. These appear in journals including Neuron, Molecular Neurodegeneration, Science Advances, Brain, Science of Signaling, Scientific Reports, Frontiers in Aging Neuroscience, Science of the Total Environment, Pediatric Research, Biochemical and Biophysical Research Communications, and bioRxiv. Research fields span Parkinson's disease, neurodegeneration, mitochondrial biogenesis, hepatocellular carcinoma, Alzheimer's disease, adipogenesis, and bronchopulmonary dysplasia.
| Species | Application | Title |
|---|---|---|
Mol Neurodegener Preclinical studies and transcriptome analysis in a model of Parkinson's disease with dopaminergic ZNF746 expression | ||
Neuron Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease.
| ||
Sci Adv Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease. | ||
Brain Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration. | ||
Brain Parkin interacting substrate zinc finger protein 746 is a pathological mediator in Parkinson's disease. | ||
Sci Total Environ Manganese-induced PINK1 S-nitrosylation exacerbates nerve cell damage by promoting ZNF746 repression of mitochondrial biogenesis |
