Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human testis tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24563-1-AP targets NCAPG in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19926 Product name: Recombinant human NCAPG protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 664-1015 aa of BC068467 Sequence: TLHCEGTEINSDDEQESKEVEETATAKNVLKLLSDFLDSEVSELRTGAAEGLAKLMFSGLLVSSRILSRLILLWYNPVTEEDVQLRHCLGVFFPVFAYASRTNQECFEEAFLPTLQTLANAPASSPLAEIDITNVAELLVDLTRPSGLNPQAKTSQDYQALTVHDNLAMKICNEILTSPCSPEIRVYTKALSSLELSSHLAKDLLVLLNEILEQVKDRTCLRALEKIKIQLEKGNKEFGDQAEAAQDATLTTTTFQNEDEKNKEVYMTPLRGVKATQASKSTQLKTNRGQRKVTVSARTNRRCQTAEADSESDHEVPEPESEMKMRLPRRAKTAALEKSKLNLAQFLNEDLS Predict reactive species |
| Full Name | non-SMC condensin I complex, subunit G |
| Calculated Molecular Weight | 1015 aa, 114 kDa |
| Observed Molecular Weight | 131 kDa |
| GenBank Accession Number | BC068467 |
| Gene Symbol | NCAPG |
| Gene ID (NCBI) | 64151 |
| RRID | AB_2879610 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BPX3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NCAPG antibody 24563-1-AP | Download protocol |
| IHC protocol for NCAPG antibody 24563-1-AP | Download protocol |
| IP protocol for NCAPG antibody 24563-1-AP | Download protocol |
| WB protocol for NCAPG antibody 24563-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NCAPG antibody (Cat# 24563-1-AP) has 12 citations. These appear in journals including J Nanobiotechnology, Cell Death & Disease, J Transl Med, Breast Cancer Res, Int J Mol Sci, Front Pharmacol, Sci Rep, J Cancer, Front Oncol, J Gastrointest Oncol, and Int J Biol Sci. Research fields span oncology—covering breast, prostate, hepatocellular, neuroblastoma, glioma, colorectal, endometrioid, and lung cancers—plus liver regeneration and immune regulation, with applications in WB, IHC, IF, and CoIP.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Hepatocyte-derived tissue extracellular vesicles safeguard liver regeneration and support regenerative therapy | ||
Cell Death Dis NCAPG confers trastuzumab resistance via activating SRC/STAT3 signaling pathway in HER2-positive breast cancer.
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J Transl Med Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance | ||
J Transl Med NCAPG promotes the proliferation of hepatocellular carcinoma through the CKII-dependent regulation of PTEN.
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Breast Cancer Res Integrated multi-omics analysis and functional validation reveal the role of TRIM2 as a potential novel biomarker in breast cancer. | ||
Int J Mol Sci Identification of NCAPG as an Essential Gene for Neuroblastoma Employing CRISPR-Cas9 Screening Database and Experimental Verification
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