Tested Applications
| Positive IHC detected in | mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24648-1-AP targets SPRR2F in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20323 Product name: Recombinant human SPRR2F protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-54 aa of BC156788 Sequence: VCPAPKCPEPCPPPKCPEPCPPSKCPQSCPPQQCQQKC Predict reactive species |
| Full Name | small proline-rich protein 2F |
| Calculated Molecular Weight | 72 aa, 8 kDa |
| GenBank Accession Number | BC156788 |
| Gene Symbol | SPRR2F |
| Gene ID (NCBI) | 6705 |
| RRID | AB_2879655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RM1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPRR2F antibody 24648-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SPRR2F Polyclonal antibody (Cat# 24648-1-AP) has 2 citations. One appears in Cell Death & Disease (IF 12.2), studying urothelial cell heterogeneity and regeneration after cyclophosphamide-induced bladder injury. The other is in Materials Today Bio (IF 11), investigating 3D-printed hydrogels for inflammation resolution and vascular regeneration in infected wounds. Both citations use the antibody for immunofluorescence (IF) in human samples, spanning research fields of bladder biology and tissue engineering/wound healing.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Single-cell analysis reveals urothelial cell heterogeneity and regenerative cues following cyclophosphamide-induced bladder injury. | ||
Mater Today Bio 3D-printed PRP-infused double-network hydrogels orchestrate inflammation resolution and vascular regeneration in infected wounds. |
