Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, Jurkat cells, K-562 cells, NIH/3T3 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24716-1-AP targets PFAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20162 Product name: Recombinant human PFAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 989-1338 aa of BC167158 Sequence: MVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLSINARNWTLEGSC Predict reactive species |
| Full Name | phosphoribosylformylglycinamidine synthase |
| Calculated Molecular Weight | 1338 aa, 145 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC167158 |
| Gene Symbol | PFAS |
| Gene ID (NCBI) | 5198 |
| RRID | AB_3669446 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15067 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PFAS antibody 24716-1-AP | Download protocol |
| IHC protocol for PFAS antibody 24716-1-AP | Download protocol |
| WB protocol for PFAS antibody 24716-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PFAS Polyclonal antibody (Cat# 24716-1-AP) has 2 total citations. These appear in the European Journal of Pharmacology and PLoS Pathogens. The associated research fields include purine metabolism and hyperuricemia (gut microbiota modulation), as well as viral replication mechanisms involving host purine biosynthetic networks in neurons.
| Species | Application | Title |
|---|---|---|
Eur J Pharmacol Oxyberberine alleviates fructose-induced hyperuricemia by modulating purine metabolism and gut microbiota | ||
PLoS Pathog Japanese encephalitis virus hijacks the host purine biosynthetic network to promote viral replication in neurons. |
