Tested Applications
| Positive WB detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24728-1-AP targets MRPL47 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11744 Product name: Recombinant human MRPL47 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-230 aa of BC032522 Sequence: MAAAGLALLCRRVSSALKSSRSLITPQVPACTGLLHTTLSRKGLEEFFDDPKNWGQEKVKSGAAWTCQQLRNKSNEDLHKLWYVLLKERNMLLTLEQEAKRQRLPMPSPERLDKVVDSMDALDKVVQEREDALRLLQTGQERARPGAWRRDIFGRIIWHKFKQWVIPWHLNKRYNRKRFFALPYVDHFLRLEREKRARIKARKENLERKKAKILLKKFPHLAEAQKSSLV Predict reactive species |
| Full Name | mitochondrial ribosomal protein L47 |
| Calculated Molecular Weight | 230 aa, 27 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC032522 |
| Gene Symbol | MRPL47 |
| Gene ID (NCBI) | 57129 |
| RRID | AB_2879692 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD33 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL47 antibody 24728-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MRPL47 Polyclonal antibody (Cat# 24728-1-AP) has 1 total citation. It was used in a Western blot study published in Nature Structural & Molecular Biology (2024), investigating ribosome assembly in human mitochondria. The cited research falls within the fields of mitochondrial biology and ribosome biogenesis.
| Species | Application | Title |
|---|---|---|
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The user tested it on fibroblasts at a 1/1000 dilution and reported a clear band at approximately 27 kDa, consistent with the expected molecular weight. Overall, the feedback indicates reliable performance with a distinct, specific signal.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Manon (Verified Customer) (09-25-2025) | Clear band around 27 kDa
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