Tested Applications
| Positive WB detected in | HeLa cells, fetal human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24729-1-AP targets EFTUD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20371 Product name: Recombinant human EFTUD1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 772-1120 aa of BC156743 Sequence: LIRSMEQLTSSLNEGENTHMIHQKTQEKIWEFKGKLEQHLTGRRWRNIVDQIWSFGPRKCGPNILVNKSEDFQNSVWTGPADKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQGASDLAKEGQEENETCSGGNENQELQDGCSEAFEKRTSQKGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATGDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK Predict reactive species |
| Full Name | elongation factor Tu GTP binding domain containing 1 |
| Calculated Molecular Weight | 1120 aa, 125 kDa |
| Observed Molecular Weight | 125-135 kDa |
| GenBank Accession Number | BC156743 |
| Gene Symbol | EFTUD1 |
| Gene ID (NCBI) | 79631 |
| RRID | AB_2879693 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z2Z2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EFTUD1 (Elongation Factor Tu GTP Binding Domain Containing Protein 1) is a protein involved in ribosome biogenesis and translation activation. It is a member of the GTP-binding elongation factor family. EFTUD1 plays a crucial role in the release of eukaryotic translation initiation factor 6 (eIF6) from 60S ribosome subunits, allowing these subunits to join with 40S subunits to form actively translating 80S complexes. (PMID: 25015090)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EFTUD1 antibody 24729-1-AP | Download protocol |
| WB protocol for EFTUD1 antibody 24729-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EFTUD1 Polyclonal antibody (Cat# 24729-1-AP) has a total of 1 citation. The cited publication appeared in Molecular Medicine (Impact Factor 8.3), published in 2026. The research field is ribosome biogenesis, specifically investigating the functional impact of the eIF6 N106S mutation in wild-type and Shwachman-Diamond syndrome cells, using Western blot as the application.
| Species | Application | Title |
|---|---|---|
Mol Med Functional impact of the eIF6 N106S mutation on ribosome biogenesis in wild type and Shwachman-Diamond syndrome cells. |
