Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, MDA-MB-453s cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue, human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24787-1-AP targets PHF14 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20542 Product name: Recombinant human PHF14 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 536-885 aa of BC152414 Sequence: RINARLQQYRAKAELARSTRPQAWVPREKLPRPLTSSASAIRKLMRKAELMGISTDIFPVDNSDTSSSVDGRRKHKQPALTADFVNYYFERNMRMIQIQENMAEQKNIKDKLENEQEKLHVEYNKLCESLEELQNLNGKLRSEGQGIWALLGRITGQKLNIPAILRAPKERKPSKKEGGTQKTSTLPAVLYSCGICKKNHDQHLLLLCDTCKLHYHLGCLDPPLTRMPRKTKNSYWQCSECDQAGSSDMEADMAMETLPDGTKRSRRQIKEPVKFVPQDVPPEPKKIPIRNTRTRGRKRSFVPEEEKHEERVPRERRQRQSVLQKKPKAEDLRTECATCKGTGDNENLVR Predict reactive species |
| Full Name | PHD finger protein 14 |
| Calculated Molecular Weight | 888 aa, 100 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC152414 |
| Gene Symbol | PHF14 |
| Gene ID (NCBI) | 9678 |
| RRID | AB_2879724 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94880 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PHF14 also named as KIAA0783 or PHD finger protein 14 is 888 amino acid protein, which contains 2 PHD-type znic fingers. PHF14 localizes in nucleus and may be involved in the regulation of transcription, DNA dependent. The function of PHF14 is still non-known.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PHF14 antibody 24787-1-AP | Download protocol |
| IP protocol for PHF14 antibody 24787-1-AP | Download protocol |
| WB protocol for PHF14 antibody 24787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PHF14 antibody (Cat# 24787-1-AP) has 8 citations spanning journals including Nature Communications, Cell Discovery, Nucleic Acids Research, Molecular Cancer Research, Clinical and Experimental Medicine, Biomedical Research International, eLife, and Oncotarget. Research fields cover pancreatic cancer stem cells, lung adenocarcinoma metastasis, TGF-β and Hippo signaling, esophageal and gastric cancers, nasopharyngeal carcinoma, chromatin remodeling, and hypoxia-responsive epigenetic regulation. Applications include WB, CoIP, ChIP, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics | ||
Cell Discov PHF14 enhances DNA methylation of SMAD7 gene to promote TGF-β-driven lung adenocarcinoma metastasis | ||
Nucleic Acids Res The high mobility group protein HMG20A cooperates with the histone reader PHF14 to modulate TGFβ and Hippo pathways
| ||
Mol Cancer Res SP4 facilitates esophageal squamous cell carcinoma progression by activating PHF14 transcription and Wnt/β-Catenin signaling
| ||
Clin Exp Med Integrated machine learning and single-cell analysis identify chromatin-remodeling gene signature for diagnosis and prognosis in nasopharyngeal carcinoma.
| ||
Biomed Res Int PHF14 Promotes Cell Proliferation and Migration through the AKT and ERK1/2 Pathways in Gastric Cancer Cells.
|
Reviews
What customers say
The single review for this antibody is a 5-star rating. The user tested it on MDA-MB-231, HEK293T, and MCF7 cells for Western Blot and Immunoprecipitation at a 1:500 dilution. They reported good results in WB, IP, and co-IP, and confirmed via siRNA knockdown that the identified band specifically corresponds to PHF14. Overall, the reviewer found the antibody reliable and specific for its intended applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jose C. (Verified Customer) (10-14-2019) | The antibody gives good results in WB, IP and co-IP. We have verified by siRNA that the identified band correspond to PHF14.
 |
