Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24790-1-AP targets GIGYF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20329 Product name: Recombinant human GIGYF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 777-1104 aa of BC146775 Sequence: KRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSNADTKNSNMGFWDDAV Predict reactive species |
| Full Name | GRB10 interacting GYF protein 2 |
| Calculated Molecular Weight | 1320 aa, 152 kDa |
| Observed Molecular Weight | 150-170 kDa |
| GenBank Accession Number | BC146775 |
| Gene Symbol | GIGYF2 |
| Gene ID (NCBI) | 26058 |
| RRID | AB_2879727 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6Y7W6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GIGYF2 (GRB10 interacting GYF protein 2) was initially identified through its interaction with GRB10, an adapter protein that binds activated INS-like growth factor (IGF-I) and INS receptors (PMID: 12771153; 19744960). It may act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. The gene of GIGYF2 has been proposed as the Parkinson-disease (PD) gene underlying the PARK11 locus (PMID: 18358451).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GIGYF2 antibody 24790-1-AP | Download protocol |
| IHC protocol for GIGYF2 antibody 24790-1-AP | Download protocol |
| IP protocol for GIGYF2 antibody 24790-1-AP | Download protocol |
| WB protocol for GIGYF2 antibody 24790-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GIGYF2 Polyclonal antibody (Cat# 24790-1-AP) has 16 citations. These appear in journals including Science, Nature Communications, Nucleic Acids Research, Cell Reports, Redox Biology, Cell Death & Disease, Molecular Medicine, Biochemical Pharmacology, iScience, Biomedicines, Journal of Cell Science, and PNAS. Research fields span mRNA translation and stability, ribosome quality control, RNA-binding protein function, vascular aging, hepatic insulin resistance, colorectal cancer, and viral biology (SARS-CoV-2, HCV, vaccinia virus). Applications include WB, IF, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Redox Biol Aberrant hyper-expression of the RNA binding protein GIGYF2 in endothelial cells modulates vascular aging and function
| ||
Nucleic Acids Res SARS-CoV-2 Nsp2 recruits GIGYF2 near viral replication sites and supports viral protein production | ||
Nucleic Acids Res Ribosome Quality Control mitigates the cytotoxicity of ribosome collisions induced by 5-Fluorouracil |
Reviews
What customers say
Users report that this GIGYF2 antibody performs adequately for Western Blot. One reviewer noted the bands are not the sharpest, while another found the overall quality good with acceptable specificity, though some non-specific bands appeared near the predicted GIGYF2 size. The latter user confirmed correct band identification using knockout cells and used a 1:1000 dilution in HEK293 cells. Overall, the antibody is considered functional with good specificity, though band sharpness and minor non-specific signals may require optimization.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aditya (Verified Customer) (10-08-2024) | works okay, bands are not the sharpest
|
Parisa (Verified Customer) (09-26-2023) | Good qulity, we could see some other non-specific bands close to the predicted GIGYF2 size and we could tell which one is GIGYF2 using the KO cells. But the antibody has a good specificity.
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